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产品描述
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Storage
- Temperature: Store at -80°C for long-term stability.
- Conditions: Avoid repeated freeze-thaw cycles to maintain vector integrity.
- Stability: When stored under recommended conditions, the vector remains stable for up to 12 months.
- Handling: Thaw on ice before use and keep on ice during handling to prevent degradation.
Product Description
- Overview: The pMYs-IRES-Neo Retroviral Vector is designed for efficient gene delivery and stable expression in mammalian cells. This vector utilizes retroviral mechanisms for integration into the host genome, facilitating long-term expression of the gene of interest.
- Components: Incorporates an Internal Ribosome Entry Site (IRES) for bicistronic expression, allowing simultaneous expression of a gene of interest and the neomycin resistance gene.
- Safety: Non-replicative, ensuring safety in laboratory settings.
Key Features
- High Efficiency: Optimized for high transduction efficiency in a variety of mammalian cell lines.
- Stable Integration: Provides stable gene expression through integration into the host genome.
- Selectable Marker: Contains a neomycin resistance gene for easy selection of successfully transduced cells.
- Bicistronic Expression: Enables simultaneous expression of two genes, enhancing experimental versatility.
What's Included
- Vector Plasmid: A vial containing the pMYs-IRES-Neo Retroviral Vector plasmid DNA.
- User Manual: Detailed instructions for handling, transfection, and selection processes.
- Technical Support: Access to expert support for troubleshooting and optimization.
Applications
- Gene Therapy Research: Ideal for studies involving gene therapy, allowing exploration of gene function and therapeutic potential.
- Stable Cell Line Generation: Useful for creating cell lines with consistent gene expression over time.
- Functional Genomics: Facilitates the study of gene function and regulation in a controlled environment.
- Drug Resistance Studies: Allows investigation into mechanisms of drug resistance via neomycin selection.
By providing detailed information on these aspects, you ensure users have a comprehensive understanding of the product's utility and handling requirements, enhancing its application in research settings.
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