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产品描述
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Certainly! Here's a detailed supplement and improvement of the product information for the pMXs-IRES-Neo Retroviral Vector across the specified aspects:
Storage
- Temperature: Store the retroviral vector at -80°C to ensure stability and integrity. Avoid repeated freeze-thaw cycles.
- Conditions: Keep the product in a tightly sealed container to prevent contamination. Minimize exposure to light and moisture.
- Shelf Life: When stored correctly, the vector remains stable for up to 12 months from the date of receipt.
Product Description
The pMXs-IRES-Neo Retroviral Vector is a versatile tool designed for efficient gene delivery into mammalian cells. This vector is derived from Moloney Murine Leukemia Virus (MMLV) and is engineered to include an internal ribosome entry site (IRES) followed by a neomycin resistance gene. The IRES allows for the co-expression of a gene of interest along with the selection marker, facilitating stable integration and expression in host cells.
Key Features
- High Efficiency: Optimized for efficient gene transfer and stable integration into the host genome.
- Dual Expression: Features an IRES element for simultaneous expression of the gene of interest and the neomycin resistance gene.
- Safety: Designed with safety features to reduce the risk of generating replication-competent retrovirus.
- Versatile Applications: Suitable for gene function studies, protein expression, and generation of stable cell lines.
What's Included
- pMXs-IRES-Neo Retroviral Vector: Provided in a concentrated solution for easy handling and transduction.
- User Manual: Detailed instructions for vector handling, transduction protocols, and troubleshooting tips.
- Certificate of Analysis: Verification of quality, concentration, and purity of the vector.
- Technical Support: Access to expert advice for optimizing experimental conditions and protocols.
Applications
- Gene Function Studies: Use the vector to investigate gene function and regulation in various mammalian cell types.
- Stable Cell Line Generation: Create cell lines expressing your gene of interest, suitable for long-term studies and assays.
- Protein Expression: Efficiently express proteins for functional assays, therapeutic research, or structural analyses.
- Drug Screening: Utilize the vector in high-throughput screening assays to evaluate drug effects on gene expression and cellular pathways.
This comprehensive information aims to assist researchers in effectively utilizing the pMXs-IRES-Neo Retroviral Vector for their scientific studies.
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