RTV-006,pBABE-Puro-hTERT Retroviral Vector,Cell Biolabs

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Storage

- Temperature Requirements: Store the vector at -80°C for long-term stability. If frequent access is needed, aliquot the product and store at -20°C to minimize freeze-thaw cycles.
- Avoid Repeated Freeze-Thaw: Limit freeze-thaw cycles to preserve vector integrity and effectiveness.
- Shelf Life: Under recommended storage conditions, the vector remains stable for up to 12 months.

Product Description

- Overview: The pBABE-Puro-hTERT Retroviral Vector is engineered for the delivery and expression of the human telomerase reverse transcriptase (hTERT) gene in target cells. Designed for use in retroviral transduction, this vector is ideal for creating immortalized cell lines by extending their replicative lifespan.
- Vector Backbone: pBABE is a well-established retroviral vector system that includes a puromycin resistance gene for selection.
- Gene of Interest: hTERT, the catalytic subunit of human telomerase, involved in cellular immortality and aging research.

Key Features

- High Efficiency: Facilitates efficient gene delivery and integration into host genomic DNA.
- Stable Expression: Ensures long-term expression of hTERT in transduced cells.
- Selectable Marker: Contains a puromycin resistance gene, enabling easy selection of successfully transduced cells.
- Broad Tropism: Capable of infecting a wide range of mammalian cells, depending on packaging cell line used.

What's Included

- Vector DNA: A specified quantity of pBABE-Puro-hTERT DNA (e.g., 10 µg) in a Tris-EDTA buffer.
- Usage Instructions: Detailed protocol for retroviral packaging, infection, and selection of transduced cells.
- Safety Data Sheet (SDS): Comprehensive safety information for handling and disposal.

Applications

- Cell Line Immortalization: Create stable cell lines for extended research applications by expressing hTERT.
- Cancer Research: Investigate the role of telomerase in cancer cell proliferation and potential therapeutic interventions.
- Aging Studies: Study the effects of hTERT on cellular aging and senescence processes.
- Gene Therapy Research: Explore potential applications of hTERT delivery in regenerative medicine and gene therapy models.

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