RTV-007,pBABE-Hygro-hTERT Retroviral Vector,Cell Biolabs

Certainly! Here's a detailed description and information for the pBABE-Hygro-hTERT Retroviral Vector, including aspects such as Storage, Product Description, Key Features, What's Included, and Applications.

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pBABE-Hygro-hTERT Retroviral Vector

Storage:
- Store at -20°C for long-term storage.
- Avoid repeated freeze-thaw cycles to maintain vector integrity.
- For short-term use, aliquots can be stored at 4°C for up to one week.

Product Description:
The pBABE-Hygro-hTERT Retroviral Vector is engineered for the purpose of immortalizing cells by expressing the human telomerase reverse transcriptase (hTERT) gene. This vector utilizes the retroviral delivery system, allowing for stable integration into the host cell genome. It contains the hygromycin resistance gene, enabling selection of successfully transduced cells.

Key Features:
- hTERT Expression: Facilitates the extension of telomeres, promoting cellular immortality in various cell types.
- Hygromycin Resistance: Allows for the selection of transduced cells via hygromycin B treatment.
- Retroviral Delivery: Ensures stable integration and long-term expression of the transgene.
- Efficient Transduction: High efficiency in a broad range of cell lines, including primary cells.

What's Included:
- pBABE-Hygro-hTERT Retroviral Vector plasmid.
- Detailed protocol for plasmid amplification and retrovirus production.
- Guidelines for hygromycin selection.
- Technical support for troubleshooting and optimization.

Applications:
- Cell Immortalization: Ideal for researchers looking to extend the lifespan of primary cells or cell lines for prolonged study.
- Cancer Research: Studying the role of telomerase activity in cancer development and progression.
- Gene Function Studies: Investigating the effects of hTERT expression on cellular behavior and function.
- Biotechnology: Production of stable cell lines for protein production or other biotechnological applications.

Additional Notes:
- Ensure compliance with biosafety regulations when handling retroviral vectors.
- Consider the use of appropriate packaging cell lines for viral production to enhance transduction efficiency.
- Optimize hygromycin concentration for effective selection depending on the cell type used.

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This comprehensive information should assist you in understanding and utilizing the pBABE-Hygro-hTERT Retroviral Vector effectively in your research or applications.