RTV-005,pBABE-Neo-hTERT Retroviral Vector,Cell Biolabs

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Product Information: pBABE-Neo-hTERT Retroviral Vector


Storage
- Temperature: Store at -80°C for long-term storage.
- Avoid Freeze-Thaw Cycles: Minimize repeated freeze-thaw cycles to maintain vector integrity.
- Stability: Vectors are stable for at least 6 months when stored properly.


Product Description
The pBABE-Neo-hTERT Retroviral Vector is designed for the efficient delivery of the human telomerase reverse transcriptase (hTERT) gene into target cells. This vector is based on the pBABE retroviral backbone, which includes a neomycin resistance gene for selection in mammalian cells. The hTERT gene is commonly used for immortalizing primary cells, enabling extended cell culture and experimentation.


Key Features
- High Efficiency: Retroviral vectors are capable of high-efficiency transduction in dividing cells.
- Selective Marker: Contains a neomycin resistance gene for easy selection of transduced cells.
- Immortalization: Facilitates the expression of hTERT, aiding in the immortalization of primary cell lines.
- Integration: Allows stable integration into the host genome, ensuring long-term expression.


What's Included
- Vector DNA: A vial containing a specified concentration of the pBABE-Neo-hTERT DNA.
- Detailed Protocol: Instructions for vector preparation, transduction procedures, and selection methods.
- Quality Control Certificate: Verification of vector integrity and functional testing results.


Applications
- Cell Immortalization: Extend the lifespan of primary cells for prolonged studies.
- Gene Therapy Research: Evaluate the effects of hTERT expression in various cell types.
- Cancer Research: Study the role of telomerase in oncogenesis and cancer progression.
- Stem Cell Studies: Utilize in stem cell research to understand telomere dynamics and cellular aging.

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