RTV-001-PURO,pBABE-Puro Retroviral Vector,Cell Biolabs

Certainly! Here's a revised and comprehensive product information sheet for the pBABE-Puro Retroviral Vector:

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Product Name: pBABE-Puro Retroviral Vector

Product Description:
pBABE-Puro is a widely used retroviral vector designed for stable gene expression in mammalian cells. It is derived from the Moloney Murine Leukemia Virus (MoMLV) and includes a puromycin resistance gene, which allows for the selection of successfully transduced cells. The vector is designed to deliver and integrate a gene of interest into the host genome, facilitating long-term expression studies.

Storage:
- Store the vector at -20°C for long-term preservation.
- Avoid repeated freeze-thaw cycles to maintain vector integrity.
- For short-term usage, aliquot and store at -80°C.

Key Features:
- Puromycin Resistance: Contains a puromycin resistance gene for easy selection of transduced cells.
- High Efficiency: Capable of delivering genes into a wide range of mammalian cells with high efficiency.
- Stable Integration: Enables stable gene expression through integration into the host genome.
- Versatile Cloning Sites: Multiple cloning sites facilitate the insertion of various genes of interest.
- Retroviral Packaging: Compatible with standard retroviral packaging systems for particle production.

What's Included:
- pBABE-Puro plasmid DNA (specify concentration and volume).
- Detailed user manual with cloning and transduction protocols.
- Technical support contact information for troubleshooting and assistance.

Applications:
- Gene Expression Studies: Ideal for studying long-term gene expression effects in mammalian cell lines.
- Stable Cell Line Generation: Used to create cell lines with permanent gene expression for research and industrial applications.
- Functional Genomics: Facilitates the study of gene function and regulation by overexpressing or silencing specific genes.
- Drug Resistance Studies: Enables the investigation of puromycin resistance mechanisms and selection processes in transduced cells.
- Cancer Research: Utilized in oncogenic studies to explore the role of specific genes in tumor development and progression.

Additional Information:
- Ensure that all handling and usage comply with relevant biosafety regulations and guidelines.
- Recommended to use in conjunction with a compatible retroviral packaging cell line to generate high-titer viral particles.
- For optimal results, follow the provided protocols for transduction and selection.

For further inquiries or technical support, please contact our customer service team, who are ready to assist with any questions or additional requirements.

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This comprehensive product information should aid users in understanding and utilizing the pBABE-Puro Retroviral Vector more effectively.