RTV-002,pBABE-GFP Retroviral Vector,Cell Biolabs

Certainly! Here's an enhanced product information for the pBABE-GFP Retroviral Vector, covering various aspects:

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Product Name: pBABE-GFP Retroviral Vector

Storage:

- Temperature: Store at -20°C for long-term storage.
- Avoid: Repeated freeze-thaw cycles to maintain vector integrity.
- Stability: When stored properly, the product remains stable for at least 12 months.

Product Description:

The pBABE-GFP Retroviral Vector is a widely used tool in molecular biology for gene delivery and expression studies. This retroviral vector facilitates the stable integration of the GFP gene into the host genome, allowing for long-term expression of the green fluorescent protein. The vector is derived from the Moloney Murine Leukemia Virus (MoMLV) and is engineered to ensure efficient transduction and expression in a variety of mammalian cells.

Key Features:

- GFP Expression: Enables visualization of transduced cells through fluorescence microscopy.
- Retroviral System: Ensures stable gene integration and long-term expression.
- Broad Host Range: Compatible with a wide range of mammalian cell types.
- High Efficiency: Achieves efficient transduction rates with minimal cytotoxicity.
- Selectable Marker: May include antibiotic resistance for selection of transduced cells (verify specific vector variant).

What's Included:

- Plasmid DNA: pBABE-GFP vector DNA for transfection and packaging.
- Protocol Guide: Detailed instructions for vector use, including transfection and packaging procedures.
- Safety Data Sheet (SDS): Information on safe handling and use.

Applications:

- Gene Expression Studies: Analyze gene function by expressing GFP-tagged proteins.
- Cell Tracking: Monitor cell movement and proliferation in live cell imaging experiments.
- Reporter Assays: Use GFP as a reporter to study promoter activity and gene expression regulation.
- Gene Therapy Research: Investigate retroviral delivery systems for potential therapeutic applications.
- Stable Cell Line Creation: Develop cell lines with consistent GFP expression for various research purposes.

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This enhanced product information provides a comprehensive overview of the pBABE-GFP Retroviral Vector, ensuring users have all the necessary details to utilize the vector effectively in their research.