RTV-003,pBABE-Neo Retroviral Vector,Cell Biolabs

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pBABE-Neo Retroviral Vector


Storage
- Temperature: Store at -20°C for long-term storage.
- Conditions: Keep the vector in a tightly sealed container to prevent contamination.
- Stability: When stored properly, the vector remains stable for up to 12 months. Avoid repeated freeze-thaw cycles to maintain integrity.


Product Description
The pBABE-Neo Retroviral Vector is a widely used tool for gene delivery in mammalian cells. It is designed for high-efficiency transduction and stable integration of genetic material into the host genome. The vector contains a neomycin resistance gene, allowing for selection of successfully transduced cells using G418 antibiotic.


Key Features
- High Efficiency: Facilitates effective gene delivery and stable integration into host cell genomes.
- Selectable Marker: Neomycin resistance gene for easy selection using G418.
- Versatility: Suitable for a wide range of mammalian cell types.
- Retroviral System: Utilizes retroviral mechanisms for integration, promoting stable expression of transgenes.


What's Included
- Vector Plasmid: pBABE-Neo plasmid, provided in a lyophilized or liquid format.
- User Guide: Detailed instructions for vector handling, transfection protocols, and selection procedures.
- Certificate of Analysis: Document verifying the quality and specifications of the vector batch.


Applications
- Gene Expression Studies: Ideal for studying gene function and regulation in mammalian cells.
- Stable Cell Line Development: Create cell lines with long-term expression of transgenes.
- Cancer Research: Investigate oncogene function and tumor suppressor genes.
- Drug Resistance Assays: Use neomycin selection to study drug resistance mechanisms.
- Functional Genomics: Explore gene knockdown or overexpression effects in various cell models.

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Ensure that all handling and experimental procedures are conducted in compliance with relevant biosafety guidelines. If you have any further questions or require additional assistance, please feel free to reach out.