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产品描述
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Certainly! Here's a comprehensive product information outline for the pBABE-Hygro Retroviral Vector:
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Product Name:
pBABE-Hygro Retroviral Vector
Storage:
- Temperature: Store at -20°C.
- Conditions: Ensure the product is kept in a dry environment and tightly sealed to prevent contamination.
- Stability: The vector retains stability for up to one year under recommended storage conditions. Avoid repeated freeze-thaw cycles.
Product Description:
The pBABE-Hygro Retroviral Vector is designed for efficient gene delivery and stable integration into the host genome, utilizing retroviral transduction mechanisms. This vector contains the hygromycin resistance gene, allowing for easy selection of successfully transduced cells. It is ideal for use in mammalian systems and can be employed for both in vitro and in vivo applications.
Key Features:
- Hygromycin Resistance: Facilitates selection of transduced cells with high precision.
- Retroviral Integration: Ensures stable gene expression and long-term study potential.
- High Transduction Efficiency: Optimized for effective gene delivery across various mammalian cell lines.
- Versatile Cloning Sites: Multiple cloning sites for easy insertion of target genes.
What's Included:
- Plasmid DNA: Typically provided in a specified concentration (e.g., 1 µg/µl).
- Detailed Protocol: Comprehensive instructions for vector handling, transfection, and selection procedures.
- Technical Support: Access to expert consultation for troubleshooting and optimization.
Applications:
- Gene Function Studies: Allows researchers to investigate gene function and regulation through stable expression.
- Drug Resistance Studies: Utilizes hygromycin selection to study drug resistance mechanisms.
- Cancer Research: Facilitates insertion of oncogenes or tumor suppressor genes to study cancer progression and therapy.
- Cell Line Development: Useful in generating stable cell lines for various research and industrial applications.
- Therapeutic Gene Delivery: Potential use in developing gene therapies.
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This outline should provide comprehensive information for users considering the pBABE-Hygro Retroviral Vector for their research needs.
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