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产品描述
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Certainly! Here's a structured way to supplement and improve the product information for the pWZL-Neo Retroviral Vector, covering the mentioned aspects:
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pWZL-Neo Retroviral Vector
Storage
- Temperature: Store at -80°C to maintain stability and integrity. Avoid repeated freeze-thaw cycles.
- Conditions: Keep the vector in a tightly sealed container to prevent contamination. It is recommended to aliquot the vector for long-term storage.
- Shelf Life: When stored correctly, the vector is stable for up to 1 year.
Product Description
- The pWZL-Neo Retroviral Vector is a versatile tool designed for efficient gene delivery and stable integration into the host genome. It is commonly used for generating stable cell lines.
- Vector Backbone: Derived from Moloney Murine Leukemia Virus (MoMLV), facilitating integration into dividing cells.
- Resistance Marker: Contains a neomycin resistance gene for selection in mammalian cells.
- Size: Approximately 7.1 kb.
Key Features
- High Efficiency: Ensures robust gene delivery into target cells.
- Stable Integration: The retroviral nature allows for permanent integration of the desired gene.
- Selectable Marker: Neomycin resistance gene enables easy selection of successfully transduced cells.
- Versatility: Compatible with a wide range of mammalian cell lines.
What's Included
- pWZL-Neo Retroviral Vector: Supplied in a concentrated form suitable for transduction.
- User Manual: Detailed instructions for use, including protocols for packaging and target cell transduction.
- Quality Assurance: Certificate of analysis confirming purity and functionality.
Applications
- Stable Cell Line Generation: Ideal for creating cell lines with long-term expression of the transgene.
- Gene Function Studies: Facilitates the study of gene function through stable expression in mammalian cells.
- Therapeutic Research: Used in preclinical studies for gene therapy applications.
- Drug Resistance Studies: Investigate drug resistance mechanisms using neomycin selection.
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These enhancements provide a comprehensive overview of the pWZL-Neo Retroviral Vector, ensuring users have detailed information for optimal usage and application in their research.
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