LTV-403,pLenti-RFP-Puro Lentiviral Control Vector,Cell Biolabs

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Storage:

- Temperature: Store at -80°C for long-term preservation. Avoid repeated freeze-thaw cycles to maintain vector integrity.
- Stability: When stored properly, the lentiviral vector remains stable for at least 12 months. For short-term use, it can be stored at -20°C for up to 1 month.
- Handling: Keep on ice when thawed and use within a few hours. Aliquot the vector upon first thaw to minimize freeze-thaw cycles.

Product Description:

The pLenti-RFP-Puro Lentiviral Control Vector is a robust tool designed for the efficient delivery of genes into mammalian cells. This vector expresses the red fluorescent protein (RFP) and a puromycin resistance gene, allowing for easy visualization and selection of successfully transduced cells. Engineered for high-efficiency transduction, it is ideal for stable integration into host genomes, making it suitable for long-term studies and applications.

Key Features:

- RFP Expression: Enables fluorescence-based tracking of transduced cells.
- Puromycin Resistance: Facilitates antibiotic selection of stable cell lines.
- High Transduction Efficiency: Optimized for efficient gene delivery across a variety of mammalian cell types.
- Safety: Third-generation lentiviral system reduces the risk of replication-competent virus.
- Versatility: Suitable for both in vitro and in vivo applications.

What's Included:

- pLenti-RFP-Puro Lentiviral Vector: Provided in a ready-to-use format.
- Protocols: Detailed instructions for vector handling, transduction, and selection.
- Certificate of Analysis: Verification of product quality and concentration.

Applications:

- Gene Expression Studies: Use RFP fluorescence to monitor gene expression in real-time.
- Stable Cell Line Generation: Establish cell lines with consistent gene expression for drug screening and functional assays.
- In Vivo Studies: Suitable for animal models to study gene function and therapeutic interventions.
- Cell Tracking: Employ RFP for visualizing cell migration and proliferation in various biological contexts.
- Functional Genomics: Use for knock-in studies and assessing gene function in mammalian cells.

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