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产品描述
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Certainly! Here's a structured product information sheet for the pSMPUW-GFP-LC3 Lentiviral Expression Vector:
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Product Name:
pSMPUW-GFP-LC3 Lentiviral Expression Vector
Product Description:
The pSMPUW-GFP-LC3 Lentiviral Expression Vector is a powerful tool designed for the study of autophagy, facilitating the visualization of LC3 protein dynamics in living cells. LC3 (Microtubule-associated protein light chain 3) is a key marker in the autophagy pathway, and GFP (Green Fluorescent Protein) tagging allows real-time observation of autophagic processes. This vector is optimized for high-efficiency transduction in a wide range of mammalian cells.
Key Features:
- GFP Tagging: Enables visualization of LC3 localization and autophagic vesicle formation.
- High Transduction Efficiency: Lentiviral system ensures robust gene delivery across various mammalian cell types.
- Stable Expression: Integration into the host genome allows for stable and long-term expression.
- Versatile Applications: Suitable for in vitro and in vivo studies of autophagy.
Storage Instructions:
- Store at -80°C for long-term preservation.
- Avoid repeated freeze-thaw cycles to maintain vector integrity.
- Once thawed, aliquot into smaller volumes and store at -20°C for short-term usage.
What's Included:
- pSMPUW-GFP-LC3 Lentiviral Expression Vector (concentrated solution)
- Detailed protocol for vector transduction and expression analysis
- Certificate of Analysis (COA)
- MSDS (Material Safety Data Sheet)
Applications:
- Autophagy Research: Study LC3 dynamics and autophagic flux in real-time using GFP fluorescence.
- Cellular Imaging: Monitor autophagosome formation and maturation in live cells.
- Gene Function Studies: Investigate the role of LC3 in various cellular processes.
- Drug Screening: Evaluate compounds that modulate autophagy pathways.
- Disease Modeling: Explore autophagic mechanisms in disease contexts, including cancer and neurodegenerative disorders.
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This product information sheet is designed to provide comprehensive details to assist researchers in effectively utilizing the pSMPUW-GFP-LC3 Lentiviral Expression Vector for their studies. If you have further questions or need additional support, please contact our technical support team.
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