CBA-401,pCMV-GFP-LC3 Expression Vector,Cell Biolabs

Sure, here is a detailed product information template for the pCMV-GFP-LC3 Expression Vector:

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Product Name: pCMV-GFP-LC3 Expression Vector

Storage:
- Store at -20°C for long-term storage.
- Avoid repeated freeze-thaw cycles to maintain plasmid integrity.
- When in use, store aliquots at 4°C for short-term use.

Product Description:
The pCMV-GFP-LC3 Expression Vector is a plasmid designed for the study of autophagy, a critical cellular process involved in the degradation and recycling of cellular components. This vector incorporates the GFP (Green Fluorescent Protein) tag fused to LC3 (Microtubule-associated protein 1A/1B-light chain 3), allowing for the visualization of autophagosomes in living cells. The CMV (Cytomegalovirus) promoter ensures strong expression in a variety of mammalian cell lines, making it a versatile tool for autophagy research.

Key Features:
- High Expression Levels: Driven by the CMV promoter for robust GFP-LC3 expression.
- Fluorescent Tagging: GFP fusion enables real-time visualization of autophagy processes.
- Versatile Use: Suitable for transient or stable transfection in multiple cell types.
- Reliable Tracking: Provides a direct method to monitor autophagic flux in live cells.

What's Included:
- pCMV-GFP-LC3 Plasmid DNA: Supplied in a concentration of [specify concentration, e.g., 1 µg/µl].
- Detailed Protocol: Instructions for transfection and visualization.
- Quality Control: Certificate of analysis ensuring purity and functionality.

Applications:
- Autophagy Research: Monitor the formation and maturation of autophagosomes.
- Cell Biology Studies: Investigate cellular responses to stress and nutrient deprivation.
- Drug Screening: Evaluate compounds that modulate autophagic pathways.
- Cancer Research: Study the role of autophagy in tumor suppression and progression.
- Neuroscience: Explore the involvement of autophagy in neurodegenerative diseases.

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