CBA-312,MEF Feeder Cells (Puromycin-resistant),Cell Biolabs

Certainly! Here's a comprehensive breakdown of the product information for MEF Feeder Cells (Puromycin-resistant):

Product Description:
MEF (Mouse Embryonic Fibroblast) Feeder Cells are commonly used in cell culture to support the growth and maintenance of pluripotent stem cells, such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). These feeder cells provide essential growth factors and extracellular matrix components that help maintain the undifferentiated state of stem cells. The puromycin-resistant variant has been genetically modified to be resistant to puromycin, allowing for selective growth in the presence of this antibiotic.

Key Features:
- Puromycin Resistance: These feeder cells have been engineered to resist puromycin, allowing for easy selection and maintenance in antibiotic-containing media.
- Quality Assurance: Each batch is tested for sterility and mycoplasma contamination, ensuring a high-quality product.
- Supportive Environment: Provides an optimal growth environment for the maintenance of pluripotency in stem cell cultures.
- Consistency: Uniform growth characteristics and reliable performance across different batches.

Storage:
- Temperature: Store frozen vials at -80°C or in liquid nitrogen. It is essential to prevent repeated freeze-thaw cycles to maintain cell viability.
- Shelf Life: When stored properly, MEF Feeder Cells can remain viable for up to 6 months. It is recommended to use them as soon as possible after thawing.
- Thawing Instructions: Thaw rapidly in a 37°C water bath and immediately transfer to pre-warmed media to ensure cell viability.

What's Included:
- Cryopreserved Vial: Contains a specified number of cells (typically 1 million cells per vial).
- Certificate of Analysis: Documentation confirming sterility, viability, and puromycin resistance.
- User Guide: Detailed instructions for thawing, culturing, and maintaining the feeder cells.

Applications:
- Stem Cell Research: Ideal for supporting the growth and maintenance of ESCs and iPSCs in a laboratory setting.
- Genetic Selection: Use in experiments requiring antibiotic selection, particularly in mixed cell populations.
- Cell Differentiation Studies: Provides a controlled environment to study the differentiation potential of stem cells.
- Tissue Engineering: Supports the foundational growth of stem cells for regenerative medicine applications.

These enhanced product details provide a comprehensive understanding of MEF Feeder Cells (Puromycin-resistant), ensuring that users have all necessary information for optimal use and application in their research.