AKR-5111,HeLa/Cas9 Cell Line,Cell Biolabs

Certainly! Here's a detailed breakdown of the product information for the HeLa/Cas9 Cell Line, covering various aspects:


Product Description
The HeLa/Cas9 Cell Line is a genetically modified version of the iconic HeLa cell line, which is derived from cervical cancer cells. This variant has been engineered to stably express the Cas9 nuclease, an integral component of the CRISPR/Cas9 genome editing system. This cell line provides researchers with a robust tool for gene editing studies and functional genomics, allowing for precise manipulation of genetic material.


Storage
- Temperature: Store the cell line at -80°C for short-term storage. For long-term storage, maintain at liquid nitrogen temperatures (-196°C).
- Conditions: Cells should be kept in a cryoprotectant medium, typically containing 10% DMSO, to prevent ice crystal formation during freezing.
- Handling: Minimize freeze-thaw cycles to maintain cell viability and integrity.


Key Features
- Stable Cas9 Expression: Ensures consistent and reliable gene editing capabilities with minimal off-target effects.
- High Transfection Efficiency: Facilitates easy introduction of guide RNAs and other genetic material.
- Cancer Cell Line Background: Maintains HeLa cell characteristics, making it suitable for cancer research and drug testing.
- Versatility: Can be used for a wide range of genomic studies, including knockouts, knock-ins, and gene regulation experiments.


What's Included
- HeLa/Cas9 Cell Line Vial: A cryopreserved vial containing a specified number of cells.
- Growth Medium Recommendations: Detailed instructions for preparing the optimal growth medium to support cell propagation and maintenance.
- Certificate of Analysis: Document verifying the genetic integrity and performance specifications of the cell line.
- User Manual: Comprehensive guide on thawing, culturing, and maintaining the cell line.


Applications
- Gene Editing: Ideal for CRISPR/Cas9-mediated gene knockout and knock-in experiments.
- Functional Genomics: Study gene function and regulatory networks in cancer cells.
- Cancer Research: Explore pathways involved in cancer development and progression using a well-characterized cell line.
- Drug Discovery: Screen potential therapeutic compounds in a genetic context relevant to human cancer.

Please ensure that you follow all recommended protocols for cell culture and genetic manipulation to maintain the integrity of the HeLa/Cas9 Cell Line. Additionally, handle all biological materials in accordance with relevant safety guidelines and regulatory requirements.