AKR-5104,NIH3T3/Cas9 Cell Line,Cell Biolabs

Certainly! Here's a detailed product information template for the NIH3T3/Cas9 Cell Line, covering various aspects:

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NIH3T3/Cas9 Cell Line


Storage
- Temperature: Store at -80°C for short-term storage or in liquid nitrogen for long-term preservation.
- Conditions: Ensure cells are stored in a cryoprotectant solution, such as 10% DMSO in FBS, to prevent ice crystal formation.
- Handling: Minimize freeze-thaw cycles to maintain cell viability and integrity.


Product Description
The NIH3T3/Cas9 cell line is a genetically modified version of the NIH3T3 mouse fibroblast cell line, engineered to stably express the CRISPR-associated protein 9 (Cas9). This cell line is ideal for gene-editing applications, allowing researchers to perform targeted gene modifications efficiently. NIH3T3 cells are known for their robust growth and ease of transfection, making them a popular choice in molecular biology and genetic research.


Key Features
- Stable Cas9 Expression: Ensures consistent and reliable gene editing performance.
- High Transfection Efficiency: Facilitates the introduction of guide RNA (gRNA) for targeted gene editing.
- Rapid Proliferation: Supports fast experimental turnaround due to quick cell growth.
- Versatility: Suitable for a wide range of applications, including gene knockout, knock-in, and gene function studies.


What's Included
- Cell Vial: Contains a minimum of 1x10^6 viable cells per vial, suspended in cryoprotectant medium.
- Certificate of Analysis: Provides detailed information about cell line authentication, viability, and sterility.
- User Manual: Instructions for thawing, culturing, and maintaining the cell line.
- Safety Data Sheet (SDS): Includes handling precautions and safety information.


Applications
- Gene Editing and Functional Genomics: Use the NIH3T3/Cas9 cell line for efficient CRISPR/Cas9-mediated gene editing to study gene function and regulation.
- Cancer Research: Investigate oncogene and tumor suppressor gene roles by creating specific genetic modifications.
- Drug Discovery: Screen potential therapeutic compounds and study their effects on gene expression and cellular pathways.
- Regenerative Medicine: Explore gene editing techniques to enhance stem cell research and tissue engineering applications.

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