AKR-234,MCF-7/Luc Cell Line,Cell Biolabs

Certainly! Here's a detailed supplement and improvement of the product information for the MCF-7/Luc Cell Line, covering the aspects you've mentioned:

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MCF-7/Luc Cell Line

Storage:
- Temperature: Store the cell line in liquid nitrogen vapor phase or at -150°C for long-term preservation.
- Handling: Ensure that cells are stored in a cryoprotectant medium, typically containing 10% DMSO, to prevent ice crystal formation.
- Stability: Upon thawing, cells should be cultured immediately to ensure viability and optimal growth conditions.

Product Description:
- The MCF-7/Luc cell line is a derivative of the MCF-7 breast cancer cell line, engineered to express the luciferase reporter gene. This modification allows for easy monitoring of cell proliferation and viability via bioluminescence imaging.
- Originating from human breast adenocarcinoma, MCF-7 cells are estrogen receptor-positive, making them highly applicable for studies in cancer biology, particularly in hormone response and drug screening.

Key Features:
- Luciferase Expression: Facilitates real-time monitoring of cell activity and visualization in vitro and in vivo.
- Estrogen Sensitivity: Suitable for hormone-related cancer research, providing insights into estrogen receptor signaling pathways.
- Applications in Drug Screening: Enables testing of anti-cancer compounds with quantifiable bioluminescent output.
- Versatility: Can be used in various experimental setups, including 2D culture and 3D spheroid models.

What's Included:
- Cryovial of MCF-7/Luc Cells: Contains a specified number of cells (typically 1 million cells per vial) suspended in cryoprotectant medium.
- Certificate of Analysis: Provides detailed information on cell viability, purity, and confirmation of luciferase expression.
- Handling Instructions: Guidelines on thawing, culturing, and maintaining cells for optimal growth and experimental outcomes.

Applications:
- Cancer Research: Ideal for studying breast cancer biology, including proliferation, metastasis, and therapeutic responses.
- In Vivo Imaging: Use in animal models to track tumor growth and response to treatment via non-invasive bioluminescent imaging.
- Drug Discovery: Screening for potential anti-cancer drugs and assessing their efficacy using bioluminescent readouts.
- Hormone Studies: Investigating estrogen receptor-mediated pathways and testing hormone-based therapies.

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These sections provide a comprehensive overview of the MCF-7/Luc cell line, highlighting its uses and storage requirements. Adjustments can be made based on specific laboratory needs or experimental protocols.