AKR-214,NIH3T3/GFP Cell Line,Cell Biolabs

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NIH3T3/GFP Cell Line Product Information

Storage:
- Store the NIH3T3/GFP cell line in liquid nitrogen vapor phase for long-term preservation. Ensure the temperature remains below -150°C.
- Upon receipt, immediately transfer cells to appropriate storage conditions to maintain viability.
- Avoid repeated freeze-thaw cycles as they can compromise cell integrity and GFP expression.

Product Description:
- The NIH3T3/GFP cell line is derived from the mouse embryonic fibroblast NIH3T3 cells, genetically modified to express the Green Fluorescent Protein (GFP).
- These cells are valuable for research involving gene expression, cellular imaging, and fluorescence-based assays.
- The GFP expression allows for easy visualization under fluorescence microscopy, facilitating tracking and analysis of cellular processes.

Key Features:
- Stable GFP Expression: Cells exhibit stable and robust GFP expression, ideal for fluorescence imaging and live-cell analysis.
- High Viability: Maintains high viability post-thaw, ensuring reliable experimental results.
- Easy Cultivation: Compatible with standard NIH3T3 cell culture conditions, making integration into existing workflows seamless.
- Versatile Applications: Suitable for a range of applications including transfection studies, drug screening, and cell signaling research.

What's Included:
- Cryovial containing approximately 1 million viable NIH3T3/GFP cells.
- Certificate of Analysis detailing cell line authenticity and mycoplasma test results.
- User manual with guidelines for thawing, culturing, and maintaining optimal GFP expression.

Applications:
- Fluorescence Imaging: Use the GFP fluorescence to study cellular morphology and dynamics in real-time.
- Gene Expression Studies: Evaluate promoter activity and gene regulation using GFP as a reporter.
- Drug Screening and Development: Assess compound effects on cell viability and GFP expression as a readout for cytotoxicity or efficacy.
- Cancer Research: Investigate fibroblast behavior in tumor microenvironment studies and cell signaling pathways.
- Transfection Efficiency: Monitor transfection success and clone selection processes via GFP visualization.

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This enhanced product description provides comprehensive information for researchers looking to utilize the NIH3T3/GFP cell line in their work.