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产品描述
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Certainly! Here's a detailed product information guide for the HeLa/GFP Cell Line, covering the aspects you've mentioned:
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HeLa/GFP Cell Line Product Information
Storage:
- Temperature: Store frozen vials in liquid nitrogen vapor phase at -150°C or lower immediately upon arrival.
- Handling: Keep cells on dry ice during transport. Once thawed, maintain cells at 37°C in a humidified incubator with 5% CO₂.
- Shelf Life: Indefinite when stored correctly in liquid nitrogen.
Product Description:
- Source: Derived from the HeLa cell line, originally established from cervical cancer cells in 1951.
- Modification: Engineered to express Green Fluorescent Protein (GFP) for easy visualization and tracking.
- Morphology: Epithelial-like, adherent cells.
- Genetic Profile: Contains GFP gene under a constitutive promoter for continuous expression.
Key Features:
- Fluorescent Marker: GFP expression allows for real-time monitoring and imaging of live cells.
- Robust Growth: HeLa cells are known for their rapid proliferation and durability in various conditions.
- Versatility: Suitable for a wide range of applications, including cancer research and gene expression studies.
- Compatibility: Can be cultured in standard HeLa media and conditions.
What's Included:
- Cryopreserved Vial: Approximately 1 million cells per vial.
- Certificate of Analysis: Confirming cell line identity and GFP expression.
- Handling Instructions: Detailed guidelines for thawing, culturing, and maintaining cells.
Applications:
- Cancer Research: Ideal for studying cancer cell biology, drug testing, and cancer treatment efficacy.
- Fluorescence Imaging: GFP expression enables applications in fluorescence microscopy and flow cytometry.
- Gene Expression Studies: Analyze the effects of genetic modifications and treatments on cellular behavior.
- Cellular Tracking: Monitor cell migration and proliferation in real-time in various environments.
- Educational Use: Excellent for teaching purposes in demonstrating cell culture techniques and fluorescent imaging.
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Additional Notes:
- Safety Precautions: Handle all cell lines as potentially biohazardous materials. Use appropriate PPE and follow institutional guidelines.
- Quality Control: Each batch undergoes rigorous testing to ensure consistent GFP expression and cell viability.
- Technical Support: Available for troubleshooting and optimizing experimental setups.
For further assistance or inquiries, please contact our technical support team.
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This information should provide a comprehensive overview of the HeLa/GFP Cell Line and its potential uses in research and education.
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