AKR-209,A549/GFP Cell Line,Cell Biolabs

Certainly! Here's a detailed description and information for the A549/GFP Cell Line, focusing on Storage, Product Description, Key Features, What's Included, and Applications:

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A549/GFP Cell Line

Product Description:
The A549/GFP cell line is a derivative of the standard A549 human lung carcinoma cell line, genetically modified to express the Green Fluorescent Protein (GFP). This modification allows for easy visualization and tracking of cells under fluorescent microscopy, making it an ideal model for various research applications including cancer studies, drug screening, and gene expression analysis.

Storage:
- Temperature: Store the cells in liquid nitrogen vapor phase for long-term preservation.
- Conditions: Ensure the cells are kept at temperatures below -150°C to maintain viability.
- Handling: Upon thawing, cells should be cultured immediately to ensure optimal recovery and viability.

Key Features:
- Fluorescent Marker: Expresses GFP, facilitating real-time observation of cellular processes.
- Human Lung Carcinoma Origin: Provides a relevant model for lung cancer research.
- Stable Expression: GFP is stably integrated, ensuring consistent fluorescence across passages.
- Versatile Use: Suitable for a wide array of applications including cytotoxicity assays and transfection studies.

What's Included:
- Cryovial of A549/GFP Cells: Contains a specified number of viable cells ready for culture.
- Certificate of Analysis: Documentation verifying the identity, purity, and viability of the cell line.
- Handling Instructions: Guidelines for thawing, culturing, and maintaining the cell line.
- Safety Data Sheet (SDS): Information on safe handling and potential hazards.

Applications:
- Cancer Research: Study tumorigenic properties and response to anti-cancer drugs.
- Drug Screening: Evaluate pharmaceutical compounds for efficacy and toxicity.
- Gene Expression Studies: Use GFP as a reporter to track gene activity and regulation.
- Cell Biology: Investigate cell signaling pathways, apoptosis, and proliferation.
- Microscopy and Imaging: Utilize fluorescence microscopy to visualize cellular dynamics.

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By supplementing the product information with these details, researchers can gain a comprehensive understanding of the A549/GFP cell line and its potential uses in their scientific work.