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产品描述
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Sure! Here's a comprehensive product information outline for the 293/GFP Cell Line:
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293/GFP Cell Line
Product Description:
The 293/GFP Cell Line is derived from human embryonic kidney (HEK) cells, stably expressing the Green Fluorescent Protein (GFP). This cell line is widely used in biomedical research for studying gene expression, protein interactions, and cellular processes. The GFP allows for easy visualization and tracking of cellular events under fluorescence microscopy.
Key Features:
- Stable GFP Expression: The cell line consistently expresses GFP, providing reliable fluorescence for various applications.
- Human Origin: Derived from HEK cells, this cell line is ideal for human-related biological and pharmaceutical research.
- Versatile Use: Suitable for transfection studies, imaging applications, and protein interaction assays.
- Easy Cultivation: The 293/GFP cells are robust and can be cultured using standard HEK cell protocols.
Storage:
- Cryopreservation: Store the cells in liquid nitrogen vapor phase at temperatures below -130°C for long-term preservation.
- Short-term Storage: For immediate use, cells can be kept at -80°C, but should be transferred to liquid nitrogen for storage longer than one week.
- Thawing: Quickly thaw cells in a 37°C water bath and immediately transfer to appropriate growth media.
What's Included:
- 293/GFP Cell Vial: Contains a specified number of viable cells for initial culture setup.
- Certificate of Analysis: Provides detailed information on cell line authentication and quality assurance.
- User Manual: Comprehensive instructions on culturing, maintenance, and applications.
- Safety Data Sheet (SDS): Information on handling and safety procedures.
Applications:
- Fluorescence Microscopy: Enables visualization of cellular processes and structures in real-time.
- Gene Expression Studies: Useful for analyzing the impact of genetic modifications or treatments on GFP expression.
- Drug Screening: Facilitates the assessment of drug effects on cell viability and protein expression.
- Protein Interaction Assays: GFP tagging allows for tracking protein-protein interactions within the cell.
- Live Cell Imaging: Ideal for studying dynamic cellular events and responses to stimuli.
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Ensure to follow recommended protocols for handling and culturing to maintain cell line integrity and performance. Always refer to the user manual and SDS for detailed instructions and safety guidelines.
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