ADV-146,Ras V12 (Constitutively Active) Recombinant Adenovirus,Cell Biolabs

Certainly! Here's a comprehensive product information guide for the Ras V12 (Constitutively Active) Recombinant Adenovirus:

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Product Name: Ras V12 (Constitutively Active) Recombinant Adenovirus

Storage:
- Temperature: Store at -80°C for long-term preservation. This ensures the stability and activity of the adenovirus.
- Handling: Minimize freeze-thaw cycles to prevent degradation. Always aliquot the product upon first thawing.
- Shelf Life: When stored properly, the product remains viable for up to 12 months.

Product Description:
This recombinant adenovirus expresses the constitutively active mutant form of the Ras protein, known as Ras V12. The Ras V12 variant is engineered to continuously activate downstream signaling pathways, bypassing normal regulatory mechanisms. This tool is ideal for studying cellular processes including proliferation, differentiation, and oncogenesis. Engineered with high purity and titer, this adenovirus effectively transduces a variety of mammalian cells.

Key Features:
- Constitutive Activity: The Ras V12 mutation leads to constant activation of Ras signaling pathways, making it a powerful tool for research in cancer biology and signal transduction.
- High Titer: The adenovirus is provided at a high concentration to ensure efficient transduction across various cell types.
- Versatility: Suitable for use in numerous mammalian cell lines, including primary cells and established cell lines.
- Purity: Produced under stringent quality controls, ensuring minimal contamination and optimal performance.

What's Included:
- Ras V12 Recombinant Adenovirus: Supplied in a volume sufficient for multiple experiments, depending on the scale and cell density.
- Certificate of Analysis: Provides detailed information on the titer, purity, and functional validation.
- Technical Datasheet: Offers guidelines for usage, transduction protocols, and safety information.

Applications:
- Cancer Research: Investigate the role of Ras signaling in tumorigenesis and cancer progression by utilizing the constitutively active Ras V12.
- Signal Transduction Studies: Dissect the pathways regulated by Ras and identify downstream effectors involved in various cellular responses.
- Drug Screening: Evaluate the impact of potential therapeutic compounds on Ras-driven pathways and identify inhibitors that can attenuate hyperactive signaling.
- Cell Biology: Study the effects of Ras activation on cellular functions such as growth, survival, and morphology.

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Note: Safety measures should be followed as per institutional guidelines when handling recombinant viruses. Proper disposal methods should be employed to prevent environmental contamination. Always wear appropriate personal protective equipment (PPE) during handling and experimentation.

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