AAV-341,AAV1-LacZ Control Virus,Cell Biolabs

Certainly! Here's a detailed description of the AAV1-LacZ Control Virus, including aspects such as Storage, Product Description, Key Features, What's Included, and Applications:

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AAV1-LacZ Control Virus

Storage:
- Temperature: Store at -80°C for optimal stability and longevity.
- Conditions: Avoid repeated freeze-thaw cycles to maintain viral integrity. Use aliquots to minimize freeze-thaw events.
- Shelf Life: When stored properly, the virus remains stable for at least 12 months from the date of receipt.

Product Description:
The AAV1-LacZ Control Virus is a recombinant adeno-associated virus serotype 1 designed for efficient transduction and expression of the LacZ reporter gene. The LacZ gene encodes the enzyme β-galactosidase, which facilitates colorimetric detection in various assays, serving as a robust tool for monitoring transgene expression and viral transduction efficiency in research applications.

Key Features:
- High Transduction Efficiency: AAV1 is known for its ability to effectively transduce a wide range of cell types, including neuronal and muscle cells.
- Robust Reporter Gene: LacZ allows for easy and reliable detection through X-gal staining, providing a visual indicator of successful transduction.
- Non-Integrating Vector: AAV vectors typically remain episomal, reducing the risk of insertional mutagenesis.
- Safety: AAV vectors are non-pathogenic and have low immunogenicity, making them suitable for in vitro and in vivo studies.
- Scalability: Suitable for both small-scale and large-scale applications due to its versatile nature.

What's Included:
- AAV1-LacZ Viral Prep: A vial containing a specified titer of AAV1-LacZ virus particles, typically ranging from 10^11 to 10^13 viral genomes per milliliter.
- Product Manual: Comprehensive instructions for use, including protocols for transduction and assays for LacZ expression.
- Safety Data Sheet (SDS): Information on safe handling and potential hazards associated with the product.

Applications:
- Gene Expression Studies: Utilize the LacZ reporter gene to study promoter activity and gene regulation.
- Transduction Efficiency Assessment: Evaluate the efficiency of viral delivery in target cell types using X-gal staining.
- Cell Line and Animal Model Development: Generate stable cell lines or transgenic animals expressing LacZ for various research applications.
- Neuroscience Research: Study neuronal pathways and gene expression in neural tissues, leveraging AAV1’s affinity for neuronal cells.
- Muscle Research: Investigate gene delivery and expression in muscle cells, capitalizing on AAV1’s proficiency in transducing muscle tissue.

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This comprehensive product information aims to enhance your understanding and utilization of the AAV1-LacZ Control Virus in your research efforts. If you have any additional questions or need further assistance, feel free to reach out!