AAV-332,scAAV2-GFP Control Virus,Cell Biolabs

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Product Information for scAAV2-GFP Control Virus


Storage
- Temperature: Store at -80°C for long-term preservation.
- Avoid: Repeated freeze-thaw cycles to maintain viral integrity.
- Shelf Life: Stable for up to 12 months when stored correctly.
- Handling: Thaw on ice before use and aliquot to minimize freeze-thaw cycles.


Product Description
- Overview: scAAV2-GFP Control Virus is a self-complementary adeno-associated virus serotype 2 (scAAV2) engineered to express Green Fluorescent Protein (GFP), used as a control in gene delivery and expression studies.
- Purpose: Designed for efficient transduction and tracking of gene expression in various mammalian cell types. The GFP marker allows for easy visualization and quantification.
- Vector Information: The virus vector carries a GFP gene under the control of a CMV promoter, ensuring robust expression in transduced cells.


Key Features
- High Efficiency: scAAV2 allows for rapid onset of gene expression due to its self-complementary nature.
- Versatile Use: Suitable for a wide range of cell types, including primary cells and established cell lines.
- Safety: Non-pathogenic and replication-deficient, making it safe for laboratory use.
- Ease of Detection: GFP expression enables straightforward monitoring using fluorescence microscopy or flow cytometry.


What's Included
- Virus Suspension: One vial containing a specified volume of high-titer scAAV2-GFP virus.
- Certificate of Analysis: Documentation verifying the titer and purity of the virus.
- User Manual: Comprehensive guide for handling, transduction protocols, and troubleshooting.


Applications
- Gene Expression Studies: Utilize GFP as a reporter to study gene expression dynamics and promoter activity.
- Transduction Efficiency Testing: Benchmark transduction efficiency in comparison to other viral vectors.
- Cell Labeling: Ideal for cell tracking and identification in mixed cell populations.
- In Vivo Studies: Suitable for animal studies requiring visualization and localization of transduced cells.
- Research and Development: Useful in preclinical research for proof-of-concept experiments in gene therapy.

This comprehensive product information should help users understand the proper handling, capabilities, and applications of the scAAV2-GFP Control Virus, ensuring effective and safe use in their experiments.