AAV-303,AAV3-GFP Control Virus,Cell Biolabs

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AAV3-GFP Control Virus Product Information


Storage
- Temperature: Store at -80°C for long-term storage.
- Stability: Stable for up to 12 months when stored properly.
- Avoid: Repeated freeze-thaw cycles to maintain viral integrity and efficacy.
- Thawing: When ready to use, thaw on ice and keep at 4°C for short-term usage.


Product Description
- Overview: The AAV3-GFP Control Virus is a recombinant adeno-associated virus serotype 3, engineered to express the Green Fluorescent Protein (GFP). This viral vector is commonly used as a control in various research applications to assess transduction efficiency and expression levels.
- Composition: Contains purified viral particles suspended in a buffered solution to ensure stability and activity.
- Concentration: Provided at a high titer, typically around 1 x 10¹² vg/mL (viral genomes per milliliter).


Key Features
- High Efficiency: Offers robust transduction efficiency across various cell types.
- Strong Fluorescence: GFP allows for easy visualization and tracking of transduced cells using fluorescence microscopy or flow cytometry.
- Versatility: Suitable for both in vitro and in vivo applications.
- Safety: AAV vectors are non-pathogenic and have a low immunogenicity profile.


What's Included
- Viral Suspension: A vial containing AAV3-GFP at specified concentration and volume.
- Certificate of Analysis: Document detailing quality control metrics such as purity, titer, and sterility.
- Usage Instructions: Comprehensive guide for optimal use, including recommended MOI (Multiplicity of Infection) for different cell types.
- Safety Data Sheet (SDS): Information regarding handling and safety precautions.


Applications
- Cellular Tracking: Use GFP expression to track cell lineage and migration in both in vitro and in vivo studies.
- Gene Expression Studies: Employ as a control to measure expression levels and transduction efficiency in comparison to experimental vectors.
- Fluorescence Imaging: Ideal for visualizing cellular processes using fluorescence microscopy.
- Functional Assays: Validate experimental conditions and protocols by ensuring the successful delivery and expression of GFP.

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