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产品描述
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Certainly! Here's a structured product information guide for a high-concentration Cas9 protein with a Nuclear Localization Signal (NLS).
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Product Information: Cas9 Protein with NLS, High Concentration
Storage
- Temperature: Store at -80°C for long-term stability.
- Buffer: Supplied in a buffer containing 50 mM Tris-HCl, 300 mM NaCl, 10% glycerol, and 1 mM DTT, pH 7.5.
- Stability: Stable for at least 6 months from the date of receipt when stored properly.
- Avoid: Repeated freeze-thaw cycles. Aliquot as needed.
Product Description
The Cas9 protein with a Nuclear Localization Signal (NLS) is a high-concentration, recombinant protein designed for precise genome editing. The NLS ensures efficient transport into the nucleus, making it ideal for applications requiring high genomic targeting efficiency. This protein is derived from *Streptococcus pyogenes* and is compatible with CRISPR-Cas9 systems.
Key Features
- High Concentration: Optimized for applications requiring minimal volumes and high activity.
- Nuclear Localization Signal (NLS): Facilitates rapid and efficient nuclear import in eukaryotic cells.
- Purity: >95% as determined by SDS-PAGE.
- Activity: Verified endonuclease activity for effective DNA cleavage.
- Versatility: Compatible with a wide range of guide RNAs (gRNAs).
What's Included
- Cas9 Protein with NLS: Provided in a high-concentration solution.
- Usage Guide: Detailed instructions for optimal usage and storage.
- Quality Control Documentation: Includes certificate of analysis and SDS.
Applications
- Genome Editing: Ideal for CRISPR-based gene knockout, knock-in, and other modifications.
- Functional Genomics: Facilitates studies of gene function and interaction.
- Gene Therapy Research: Supports the development of therapeutic interventions targeting genetic disorders.
- Biotechnology: Useful in synthetic biology and the development of genetically modified organisms (GMOs).
- Drug Discovery: Assists in the validation of drug targets through precise genome modification.
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This formulation ensures that users are well-informed about the storage requirements, product attributes, and potential applications of the Cas9 protein with NLS, thereby facilitating optimal use in various research and development settings.
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