T7 mScript™ Standard mRNA Production System,C-MSC100625,CELLSCRIPT,安诺伦(北京)生物科技有限公司

Unit Definitions
One T7 mScript Standard mRNA Production System reaction produces 60 µg of 5'-capped, 3'-poly-(A)-tailed mRNA.
One unit of RNase-Free DNase I digests 1 µg of pUC19 DNA to oligodeoxynucleotides in 10 minutesat 37°C.
One unit of ScriptCap Capping Enzyme releases 1 nmole of inorganic phosphate from GTP in 10minutes at 37°C under standard assay conditions.
One unit of ScriptCap 2'-O-Methyltransferase methylates one picomole of a control Cap 0 RNA in1 hour at 37°C under standard assay conditions.
One unit of A-Plus Poly(A) Polymerase converts 1 nmole of ATP into acid-insoluble material in 10minutes at 37°C under standard assay reaction conditions.
One unit of ScriptGuard RNase Inhibitor results in 50% inhibition of 5 ng of RNase A. Activity ismeasured by the inhibition of hydrolysis of cyclic 2',3'-CMP by RNase A.
Functional Testing
The T7 mScript Standard mRNA Production System is functionally tested under standard reactionconditions using the T7 Control Template DNA and independent transcripts. The in vitro transcriptionmodule must produce at least 60 µg of RNA from 1 µg of the T7 Control Template DNA in 15 minutesat 37°C. A-Plus Poly(A) Polymerase is functionally tested in 1X A-Plus Poly(A) Tailing Buffer with1 mM ATP and a 1.4 kb transcript. The capping module enzymes are tested independently usingnon-T7 control transcript RNA to assay for completeness of reaction.
Contaminating Activity Assays
All components of the T7 mScript Standard mRNA Production System are free of detectable RNaseand DNase activity, except for the inherent activity of the RNase-Free DNase I component.