Product Applications Protocol: Cell Seeding & Encapsulation in Collagen Protocol: Hydrogel Dehydration for SEM Protocol: Hydrogel Embedding Protocol: Live Dead Cell Viability Protocol: Immunofluorescence Staining Protocol: RNA Extraction for Cells in Hydrogel Protocol: Actin Cytoskeleton Staining Protocol: Stem Cell Culture & Differentiation Protocol: Hydrogel H&E Staining Protocol: AlamarBlue Cell Proliferation Assay on PureCol Matrigel Alternatives for Neuronal Assays Matrigel Alternatives for Migration and Wound Healing Assays Collagen Long-Term Stability and Swelling White Paper

VitroCol^® is a 3 mg/ml, type I human collagen (atelocollagen) solution for 2D and 3D cell culture, or as a collagen standard. VitroCol^® collagen is naturally secreted from human neo-natal fibroblast cells, then processed and purified to yield the naturally produced human collagen.

VitroCol^® is a 3 mg/ml, type I human collagen (atelocollagen) solution for 2D and 3D cell culture, or as a collagen standard. VitroCol^® collagen is naturally secreted from human neo-natal fibroblast cells, then processed and purified to yield the naturally produced human collagen.

VitroCol ® is a 3 mg/ml, type I human collagen (atelocollagen) solution for 2D and 3D cell culture, or as a collagen standard. VitroCol ® collagen is naturally secreted from human neo-natal fibroblast cells, then processed and purified to yield the naturally produced human collagen.

Product Description VitroCol ® human collagen is the first widely available, naturally produced purified human collagen for research purposes. VitroCol ® sets the standard for purity ( > 99% collagen content), functionality and represents the only native-like human collagen offered. VitroCol ® collagen is naturally secreted from human neo-natal fibroblast cells. The human fibroblasts are cultured in optimal conditions allowing the fibroblast to naturally and efficiently secret extracellular matrix. The extracellular matrix is then processed and purified to yield the naturally produced human collagen. VitroCol ® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. VitroCol ® is supplied at approximately 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. VitroCol® is soluble atelocollagen in 0.01 N HCI, therefore, the pH is 2. VitroCol ® is especially ideal for human cell culture systems when coating of surfaces, providing preparations of thin layers of culturing cells, or use as a solid gel. VitroCol ® human collagen is provided in user-friendly packaging for use and storage. VitroCol ® is sterile filtered and is supplied as a ready to use solution. Parameter, Testing, and Method VitroCol ® Type I Collagen #5007 Sterilization Method Filtration Extraction Method Enzyme - atelocollagen Form Solution Package Size 20 mL, 100 mL Storage Temperature 2-10°C Shelf Life Minimum of 6 months from date of receipt Collagen Concentration - Biuret 2.9-3.2 mg/mL Collagen Purity - Silver Staining > 99% pH 1.9-2.1 Kinetic Gel Test (Minutes) < 40 Gel Formation Tube Test (Minutes) < 40 Fibrillogenesis (Absorbance Units) > 0.5 Electrophoretic Pattern - Coomassie Blue Characteristic Sterility - USP modified No growth Endotoxin - LAL < 5.0 EU/mL Osmolality (mOsmo H2O/kg) < 35 Cell Attachment Assay Pass Source Human Neo-Natal Fibroblasts Hydrogel Young's Modulus E (Pa) Characteristic