Product Applications Protocol: Cell Seeding & Encapsulation in Collagen Protocol: Hydrogel Dehydration for SEM Protocol: Hydrogel Embedding Protocol: Live Dead Cell Viability Protocol: Immunofluorescence Staining Protocol: RNA Extraction for Cells in Hydrogel Protocol: Actin Cytoskeleton Staining Protocol: Stem Cell Culture & Differentiation Protocol: Hydrogel H&E Staining Protocol: AlamarBlue Cell Proliferation Assay on PureCol Read our Collagen Hydrogel Shear Modulus whitepapers Matrigel Alternatives for Neuronal Assays Matrigel Alternatives for Migration and Wound Healing Assays Collagen Long-Term Stability and Swelling White Paper The graph below demonstrates gelation kinetics of our collagen hydrogels. The collagen was neutralized according to respective protocols and placed in specialized containers within the 37°C chamber of the rheometer. Shear Modulus G' (Pa) is shown on the Y axis compared to Time (minutes) on the X axis. All of the below products are in inventory and are available for order.

RatCol for 3D gels is 25 mL of 4 mg/ml, type I rat tail collagen (telocollagen) solution for 2D coatings or 3D hydrogels. This kit for 3D gels comes with a neutralization solution for easy handling. Being telocollagen, RatCol for 3D gels will form firmer hydrogels than atelocollagen.1 Liter bottles available now!

Product Description RatCol™ Type I Acid Soluble Rat Tail Collagen contains 100 mg at a concentration of approximately 4 mg/mL in a 0.02M acetic acid solution (~pH 3). RatCol™ collagen is soluble telo -collagen. Each product includes a bottle containing 100 mg of collagen solution accompanied with a bottle of pre-formulated neutralizing solution for the formation of a collagen gel. This collagen product is provided in user-friendly packaging for use and storage. This product is sterile filtered and is supplied as a ready to use solution. This product is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel. RatCol™ collagen is suitable for applications using a variety of cell lines including hepatocytes, fibroblasts and epithelial cells. Parameter, Testing, and Method RatCol™ Type I Collagen #5153 Sterilization Method Filtration Extraction Method Acid - telocollagen Form Solution Package Size 100 mg (~25 mL) Kit Storage Temperature of Collagen 2-10°C Storage Temperature of Neutralization Solution Room Temperature Shelf Life Minimum of 6 months from date of receipt Collagen Concentration - Biuret 3.5-4.5 mg/mL Collagen Purity - Silver Staining > 99% pH 3.0-3.8 Kinetic Gel Test (Minutes) < 40 Gel Formation Tube Test (Minutes) < 40 Electrophoretic Pattern - Coomassie Blue Characteristic Sterility - USP modified No growth Endotoxin - LAL < 10.0 EU/mL Osmolality (mOsmo H2O/kg) < 35 Cell Attachment Assay Pass Source Rat Tail Tendon Hydrogel Young's Modulus E (Pa) Characteristic