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产品描述
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Unit Definitions
One unit of RNase-Free DNase I digests 1 μg of pUC19 DNA to oligodeoxynucleotides in 10 minutesat 37°C.
One unit of ScriptGuard RNase Inhibitor results in 50% inhibition of 5 ng of RNase A. Activity ismeasured by the inhibition of hydrolysis of cyclic 2',3'-CMP by RNase A.
Functional Testing
The MessageMAX T7 ARCA-Capped Message Transcription Kit is functionally tested under standardreaction conditions using the T7 Control Template DNA. The kit must produce at least 40 μg of RNAfrom 1 μg of the T7 Control Template DNA in 30 minutes at 37°C.
Contaminating Activity Assays
All components of the MessageMAX T7 ARCA-Capped Message Transcription Kit are free ofdetectable RNase and DNase activity, except for the inherent activity of the RNase-Free DNase Icomponent.
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