Product Applications Protocol: Cell Seeding & Encapsulation in Collagen Protocol: Hydrogel Dehydration for SEM Protocol: Hydrogel Embedding Protocol: Live Dead Cell Viability Protocol: Immunofluorescence Staining Protocol: RNA Extraction for Cells in Hydrogel Protocol: Actin Cytoskeleton Staining Protocol: Stem Cell Culture & Differentiation Protocol: Hydrogel H&E Staining Protocol: AlamarBlue Cell Proliferation Assay on PureCol Matrigel Alternatives for Neuronal Assays Matrigel Alternatives for Migration and Wound Healing Assays Collagen Long-Term Stability and Swelling White Paper Read our Collagen Hydrogel Shear Modulus whitepapers The graph below demonstrates gelation kinetics of our collagen hydrogels. The collagen was neutralized according to respective protocols and placed in specialized containers within the 37°C chamber of the rheometer. Shear Modulus G' (Pa) is shown on the Y axis compared to Time (minutes) on the X axis. All of the below products are in inventory and are available for order.

Nutragen^® is a 6 mg/ml, type I bovine collagen (atelocollagen) solution for 2D and 3D cell culture. Due to having a higher concentration, Nutragen^® hydrogels will be stronger than other lower concentration collagen products.

Nutragen^® is a 6 mg/ml, type I bovine collagen (atelocollagen) solution for 2D and 3D cell culture. Due to having a higher concentration, Nutragen^® hydrogels will be stronger than other lower concentration collagen products.

Nutragen ® is a 6 mg/ml, type I bovine collagen (atelocollagen) solution for 2D and 3D cell culture. Due to having a higher concentration, Nutragen ® hydrogels will be stronger than other lower concentration collagen products.

Product Description Nutragen ® collagen is known as the standard of all collagens for purity ( > 99% collagen content), functionality, and the most native-like collagen available. Nutragen ® is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency. Nutragen ® collagen is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. Nutragen ® collagen is supplied at approximately a 6 mg/mL concentration to provide an extremely firm gel. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. Nutragen ® is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0. Nutragen ® collagen is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel. Nutragen ® collagen is provided in a 50 mL volume and is contained in user-friendly packaging for use and storage. Nutragen ® is sterile filtered and is supplied as a ready to use solution. Parameter, Testing, and Method Nutragen ® Type I Collagen #5010 Sterilization Method Filtration Extraction Method Enzyme - atelocollagen Form Solution Package Size 50 mL, 1000 mL Storage Temperature 2-10°C Shelf Life Minimum of 6 months from date of receipt Collagen Concentration - Biuret 5.8-6.8 mg/mL Collagen Purity - Silver Staining > 99% pH 1.9-2.1 Kinetic Gel Test (Minutes) < 40 Gel Formation Tube Test (Minutes) < 40 Fibrillogenesis (Absorbance Units) > 0.5 Electrophoretic Pattern - Coomassie Blue Characteristic Sterility - USP modified No growth Endotoxin - LAL < 1.0 EU/mL Osmolality (mOsmo H2O/kg) < 35 Cell Attachment Assay Pass Source Bovine Hide Hydrogel Young's Modulus E (Pa) Characteristic