Product Applications Protocol: Cell Seeding & Encapsulation in Collagen Protocol: Hydrogel Dehydration for SEM Protocol: Hydrogel Embedding Protocol: Live Dead Cell Viability Protocol: Immunofluorescence Staining Protocol: RNA Extraction for Cells in Hydrogel Protocol: Actin Cytoskeleton Staining Protocol: Stem Cell Culture & Differentiation Protocol: Hydrogel H&E Staining Protocol: AlamarBlue Cell Proliferation Assay on PureCol Read our Collagen Hydrogel Shear Modulus whitepapers Matrigel Alternatives for Neuronal Assays Matrigel Alternatives for Migration and Wound Healing Assays Collagen Long-Term Stability and Swelling White Paper The graph below demonstrates gelation kinetics of our collagen hydrogels. The collagen was neutralized according to respective protocols and placed in specialized containers within the 37°C chamber of the rheometer. Shear Modulus G' (Pa) is shown on the Y axis compared to Time (minutes) on the X axis. All of the below products are in inventory and are available for order.

FibriCol^® is a 10 mg/ml, type I bovine collagen (atelocollagen) solution for 2D and 3D cell culture. Due to having a high concentration, FibriCol^® hydrogels will be firmer than other lower concentration collagen products.

FibriCol^® is a 10 mg/ml, type I bovine collagen (atelocollagen) solution for 2D and 3D cell culture. Due to having a high concentration, FibriCol^® hydrogels will be firmer than other lower concentration collagen products.

FibriCol ® is a 10 mg/ml, type I bovine collagen (atelocollagen) solution for 2D and 3D cell culture. Due to having a high concentration, FibriCol ® hydrogels will be firmer than other lower concentration collagen products.

Product Description FibriCol ® collagen is known as the standard of all collagens for purity ( > 99% collagen content), functionality, and the most native-like collagen available. FibriCol ® is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency. FibriCol ® collagen is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. FibriCol ® collagen is supplied at approximately a 10 mg/mL concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. FibriCol ® is soluble atelocollagen in 0.01 N HCI, therefore, the pH is 2. FibriCol ® collagen is ideal for applications where high concentrations of collagen are needed. FibriCol ® collagen is provided in a 20 mL volume and is contained in user-friendly packaging for use and storage. FibriCol ® is sterile filtered and is supplied as a ready to use solution. Parameter, Testing, and Method FibriCol ® Type I Collagen #5133 Sterilization Method Filtration Extraction Method Enzyme - atelocollagen Form Solution Package Size 20 mL, 100 mL Storage Temperature 2-10°C Shelf Life Minimum of 6 months from date of receipt Collagen Concentration - Biuret 9.0-11.5 mg/mL Collagen Purity - Silver Staining > 99.9% pH 1.9-2.1 Kinetic Gel Test (Minutes) < 40 Gel Formation Tube Test (Minutes) < 40 Fibrillogenesis (Absorbance Units) > 0.5 Electrophoretic Pattern - Coomassie Blue Characteristic Sterility - USP modified No growth Endotoxin - LAL < 1.0 EU/mL Osmolality (mOsmo H2O/kg) < 35 Cell Attachment Assay Pass Source Bovine Hide Hydrogel Young's Modulus E (Pa) Characteristic