Product Applications Protocol: Cell Seeding & Encapsulation in Collagen Protocol: Hydrogel Dehydration for SEM Protocol: Hydrogel Embedding Protocol: Live Dead Cell Viability Protocol: Immunofluorescence Staining Protocol: RNA Extraction for Cells in Hydrogel Protocol: Actin Cytoskeleton Staining Protocol: Stem Cell Culture & Differentiation Protocol: Hydrogel H&E Staining Protocol: AlamarBlue Cell Proliferation Assay on PureCol Read our Collagen Hydrogel Shear Modulus whitepapers Matrigel Alternatives for Neuronal Assays Matrigel Alternatives for Migration and Wound Healing Assays The graph below demonstrates gelation kinetics of our collagen hydrogels. The collagen was neutralized according to respective protocols and placed in specialized containers within the 37°C chamber of the rheometer. Shear Modulus G' (Pa) is shown on the Y axis compared to Time (minutes) on the X axis. All of the below products are in inventory and are available for order.

PureCol® EZ Gel is a 5 mg/ml ready-to-use bovine atelocollagen solution that forms a 3D hydrogel by simply warming to 37°C. This product is pH neutral and isotonic formulated with DMEM/F12 medium and ready for immediate cell addition and 3D hydrogels.

PureCol® EZ Gel is a 5 mg/ml ready-to-use bovine atelocollagen solution that forms a 3D hydrogel by simply warming to 37°C. This product is pH neutral and isotonic formulated with DMEM/F12 medium and ready for immediate cell addition and 3D hydrogels.

Product Description PureCol ® EZ Gel is a ready-to-use collagen solution that forms a firm gel by simply warming to 37°C in an incubator. The product consists of purified Type I collagen at a concentration of 0.5%, (~5 mg/ml), DMEM/F-12 medium and a mixture of L-glutamine and dipeptide (L-alanine-L-glutamine) to provide a long-lasting L-glutamine source for cell culture. PureCol ® EZ Gel is designed to improve gel consistency by pre-formulation of the medium and adjustment of the product to a neutral pH. This product avoids the inconsistencies in the preparation of the gel that can arise through variables of reagent addition, pH adjustment and handling conditions. PureCol ® EZ Gel is ideal for providing a firm gel and can also be used in the preparation of a thin layer for culturing cells. PureCol ® EZ Gel collagen is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution. Parameter, Testing, and Method PureCol ® EZ Gel Type I Collagen #5074 Sterilization Method Filtration Extraction Method Enzyme - atelocollagen Form Solution Package Size 35 mL Storage Temperature 2-10°C Shelf Life Minimum of 6 months from date of receipt Collagen Concentration - Biuret ~5 mg/mL Collagen Purity - Silver Staining > 99% pH 6.9-7.4 Kinetic Gel Test (Minutes) < 40 Gel Formation Tube Test (Minutes) < 40 Fibrillogenesis (Absorbance Units) > 0.5 Electrophoretic Pattern - Coomassie Blue Characteristic Sterility - USP modified No growth Endotoxin - LAL < 1.0 EU/mL Osmolality (mOsmo H2O/kg) 300-360 Cell Attachment Assay Pass Source Bovine Hide Hydrogel Young's Modulus E (Pa) Characteristic Medium Supplement DMEM/F-12 L-Glutamine Source Mixture of L-Glutamine and Dipeptide (L-alanine-L-glutamine)