Product Applications Protocol: Cell Seeding & Encapsulation in Collagen Protocol: Hydrogel Dehydration for SEM Protocol: Hydrogel Embedding Protocol: Live Dead Cell Viability Protocol: Immunofluorescence Staining Protocol: RNA Extraction for Cells in Hydrogel Protocol: Actin Cytoskeleton Staining Protocol: Stem Cell Culture & Differentiation Protocol: Hydrogel H&E Staining Protocol: AlamarBlue Cell Proliferation Assay on PureCol Read our Collagen Hydrogel Shear Modulus whitepapers Matrigel Alternatives for Neuronal Assays Matrigel Alternatives for Migration and Wound Healing Assays Collagen Long-Term Stability and Swelling White Paper The graph below demonstrates gelation kinetics of our collagen hydrogels. The collagen was neutralized according to respective protocols and placed in specialized containers within the 37°C chamber of the rheometer. Shear Modulus G' (Pa) is shown on the Y axis compared to Time (minutes) on the X axis. All of the below products are in inventory and are available for order.

TeloCol-10 is 20 mL of 10 mg/ml, type I bovine collagen (telocollagen) solution for 2D coatings or 3D hydrogels. TeloCol-10 has a high viscosity and concentration. Being telocollagen, TeloCol-10 polymerizes quickly and can form firm hydrogels.

Product Description TeloCol ® -10 bovine collagen solution is derived from an acid extraction process yielding a telopeptide-intact collagen. TeloCol ® is soluble telocollagen in 0.01 N HCL. The telopeptide regions at both ends of the collagen chain, N- and C-telopeptide regions, are maintained. TeloCol ® -10 collagen is approximately 95% Type I collagen with the remainder being comprised of Type III collagen. Each product includes a bottle containing 20 ml of collagen solution. This product is supplied at a concentration of approximately 10 mg/ml. TeloCol ® -10 is sterile filtered and provided in user-friendly packaging for use and storage. TeloCol ® -10 is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel. Parameter, Testing, and Method TeloCol ® -10 Type I Collagen #5226 Sterilization Method Filtration Extraction Method Acid - telocollagen Form Solution Package Size 20 mL Storage Temperature 2-10°C Shelf Life Minimum of 6 months from date of receipt Collagen Concentration - Biuret 9.0-11.0 mg/mL Collagen Purity - Silver Staining > 99% pH 1.9-3.0 Kinetic Gel Test (Minutes) < 40 Gel Formation Tube Test (Minutes) < 40 Fibrillogenesis (Absorbance Units) > 0.5 Electrophoretic Pattern - Coomassie Blue Characteristic Sterility - USP modified No growth Endotoxin - LAL < 10.0 EU/mL Osmolality (mOsmo H2O/kg) < 35 Cell Attachment Assay Pass Source Bovine Hide Hydrogel Young's Modulus E (Pa) Characteristic