Product Applications Protocol: Cell Seeding & Encapsulation in Collagen Protocol: Hydrogel Dehydration for SEM Protocol: Hydrogel Embedding Protocol: Live Dead Cell Viability Protocol: Immunofluorescence Staining Protocol: RNA Extraction for Cells in Hydrogel Protocol: Actin Cytoskeleton Staining Protocol: Stem Cell Culture & Differentiation Protocol: Hydrogel H&E Staining Protocol: AlamarBlue Cell Proliferation Assay on PureCol Read our Collagen Hydrogel Shear Modulus whitepapers The graph below demonstrates gelation kinetics of our collagen hydrogels. The collagen was neutralized according to respective protocols and placed in specialized containers within the 37°C chamber of the rheometer. Shear Modulus G' (Pa) is shown on the Y axis compared to Time (minutes) on the X axis. All of the below products are in inventory and are available for order.

Our high concentration rat tail collagen contains 10 mL of 10 mg/ml telocollagen. Being telocollagen, rat tail collagen will form firmer hydrogels than atelocollagen. Our collagen is highly purified, lot-to-lot consistent, and on-the-shelf.

Our high concentration rat tail collagen contains 10 mL of 10 mg/ml telocollagen. Being telocollagen, rat tail collagen will form firmer hydrogels than atelocollagen. Our collagen is highly purified, lot-to-lot consistent, and on-the-shelf.

Product Description RatCol™ High Concentration Type I Acid Soluble Rat Tail Collagen contains 100 mg at a concentration of approximately 10 mg/mL in a 0.02M acetic acid solution (~pH 3). RatCol™ collagen is soluble telo -collagen. This collagen product is provided in user-friendly packaging for use and storage. This product is sterile filtered and is supplied as a ready to use solution. This product is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel. RatCol™ collagen is suitable for applications using a variety of cell lines including hepatocytes, fibroblasts and epithelial cells. Parameter, Testing, and Method RatCol™ Type I Collagen #IKD119261001 Sterilization Method Filtration Extraction Method Acid - telocollagen Form Solution Package Size 100 mg (~10 mL) Kit Storage Temperature of Collagen 2-10°C Storage Temperature of Neutralization Solution Room Temperature Shelf Life Minimum of 6 months from date of receipt Collagen Concentration - Biuret ~10.0 mg/mL Collagen Purity - Silver Staining > 99% pH 3.0-3.8 Kinetic Gel Test (Minutes) < 40 Gel Formation Tube Test (Minutes) < 40 Electrophoretic Pattern - Coomassie Blue Characteristic Sterility - USP modified No growth Endotoxin - LAL < 10.0 EU/mL Osmolality (mOsmo H2O/kg) < 35 Cell Attachment Assay Pass Source Rat Tail Tendon Hydrogel Young's Modulus E (Pa) Characteristic