The 5-FAM (Green) Collagen Hybridizing Peptide (CHP) is a synthetic peptide that can specifically bind to denatured collagen strands through hydrogen bonding, both in histology, in vivo, and in vitro (3D cell culture). CHP's serve as a unique tool for identifying denatured collagen.

The 5-FAM (Green) Collagen Hybridizing Peptide (CHP) is a synthetic peptide that can specifically bind to denatured collagen strands through hydrogen bonding, both in histology, in vivo, and in vitro (3D cell culture). CHP's serve as a unique tool for identifying denatured collagen.

Product Description Collagen Hybridizing Peptides (CHP) conjugates from Advanced BioMatrix are labeled with 5-FAM (F-CHP, Catalog No. 5264-60UG) / sulfo-Cyanine3 (R-CHP, Catalog No. 5276-60UG) for fluorescence detection, or biotin (B-CHP, Catalog No. 5265-60UG) for avidin/streptavidin-mediated detection. The triple helix is the hallmark protein structure of collagen. During tissue remodeling, the triple helical collagen molecules are degraded by specific proteases (e.g., MMP or cathepsin K) and become unfolded at body temperature. The Collagen Hybridizing Peptide (CHP) is a synthetic peptide that can specifically bind to such denatured collagen strands through hydrogen bonding, both in histology, in vivo, and in vitro (3D cell culture). By sharing the structural motif and the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen strands, in a fashion that is similar to a DNA fragment annealing to its complimentary DNA strand during PCR. CHP is an extremely specific probe for unfolded collagen molecules: it has negligible affinity to intact collagen molecules due to the lack of binding sites; it is also inert towards non-specific binding because of its neutral and hydrophilic nature. Parameter, Testing, and Method CHP 5-FAM #5264 Specialty Straight-forward fluore-scence detection in green Formula C 135 H 175 N 31 O 45 Molecular Weight 2952.01 g/mol Ex/Em 494 nm / 512 nm Synonym Collagen Mimetic Peptide, CMP Solubility Aqueous Buffers Storage -20°C as powder for long term storage. 4°C after reconstitution in water. Protect from light. Source Synthetic Shelf Life Minimum of 6 months from date of receipt Storage/Stability: The product ships on ambient. Store the product at -20°C. Features of CHP reagents More informative, reliable and convenient than zymography, DQ collagen, SHG, and TEM High affinity and unparalleled specificity to collagen with essentially no nonspecific binding Applicable to denatured collagens of all subtypes and from all species; binding relying on collagen’s secondary structure instead of specific epitopes Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval A non-antibody approach with no species restrictions, compatible for co-staining with any antibody Small size (2% of IgG by MW) enabling facile tissue penetration during whole tissue staining (with no need for sectioning) Stable in solution under 4 °C, eliminating the need to aliquot for storage