Product Applications Read our Methacrylated Extracellular Matrices eBrochure Here PhotoCol ® Methacrylated Collagen is a photocrosslinkable type I collagen for 3D cell culture and bioprinting. As the most abundant protein in the body, collagen is a key component for cell culture. Collagen methacrylate can be used as a rapidly self-assembling type I collagen to form cross-linked hydrogels for tissue engineering[1]. It has been used with mesenchymal stem cells[2], fibroblasts, adipose derived stem cells, epithelial cells, and many more. Collagen methacrylate is useful for forming scaffolds with varying degree of stiffness, by altering collagen concentration or the dose of UV-light exposure[2]. It is also being extensively characterized for its usefulness in 3D bioprinting (extrusion, inkjet, and photolithographic[3]). PhotoCol ® has been used for created 3D microniches[4], as well as for 3D bioprinting human corneas[5]. References: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172302/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4243547/ http://www.worldscientific.com/doi/abs/10.1142/S2339547817500091 https://onlinelibrary.wiley.com/doi/abs/10.1002/adbi.201700237 https://www.ncbi.nlm.nih.gov/pubmed/29772228

PhotoCol^® is 100 mg of lyophilized methacrylated Type I collagen. PhotoCol^® provides native-like 3D collagen gels with the unique attributes to be prepared at various concentrations and photocrosslinked (requires a photoinitiator) to provide various gel stiffness.

PhotoCol^® is 100 mg of lyophilized methacrylated Type I collagen. PhotoCol^® provides native-like 3D collagen gels with the unique attributes to be prepared at various concentrations and photocrosslinked (requires a photoinitiator) to provide various gel stiffness.

PhotoCol ® is 100 mg of lyophilized methacrylated Type I collagen. PhotoCol ® provides native-like 3D collagen gels with the unique attributes to be prepared at various concentrations and photocrosslinked (requires a photoinitiator) to provide various gel stiffness.

Product Description Advanced BioMatrix offers PhotoCol ® , a purified methacrylated Type I bovine collagen kit. PhotoCol® provides native-like 3D collagen gels with the unique attributes to be prepared at various concentrations and crosslinked to provide various gel stiffness. PhotoCol ® kits consists of purified methacrylated Type I bovine collagen as the core component with other support reagents in the kit. This PhotoCol ® product only contains the methacrylated collagen. For the entire photocrosslinking kit, please purchase one of the "Kits." The kits each contain the first three rows shown below, accompanied by the selected photoinitiator. Table 1: Item Catalog Number Package Size Storage Temperature Methacrylated Collagen #5198 100 mg 2-10°C 20 mM Acetic Acid #5079 50 mL 2-30°C Neutralization Solution #5205 10 mL Room Temperature The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen where the collagen has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the a-amines groups on the N-termini. > 20% of the total lysine residues of the collagen molecule have been methacrylated. The collagen is extracted from bovine hide and contains a high monomer content. Parameter, Testing, and Method Methacrylated Collagen #5198 Sterilization Method Filtration Extraction Method Acid - Telocollagen Form Lyophilized Powder Degree of Methacrylation > 20% Package Size 100 mg Storage Temperature 2-10°C Shelf Life Minimum of 6 months from date of receipt Shelf Life After Reconstitution 2 months Collagen Purity - Silver Staining > 99% Kinetic Gel Test (Minutes) < 40 Gel Formation Tube Test (Minutes) < 40 Electrophoretic Pattern - Coomassie Blue Characteristic Sterility - USP modified No growth Endotoxin (LAL) < 10.0 EU/mL Source Bovine Hide Hydrogel Young's Modulus E (Pa) Characteristic