Product Applications Read our PhotoHA-Stiff Rheology Photocrosslinking White Paper here. Read our Methacrylated Extracellular Matrices eBrochure Here PhotoHA™ Methacrylated Hyaluronic acid can be used to form hydrogels for ex-vivo engineering of autologous cartilage tissue[1] or as a mesenchymal stem cell carrier in cartilage repair[2]. Because the stiffness can be widely adjusted by altering concentration or UV-light exposure, methacrylated HA has been used to measure the effects of matrix stiffness on cell phenotype and function[3][4]. Methacrylated HA can be used for 3D bioprinting (extrusion[5], inkjet[5] and photolithographic[6]) to create structures that promote osteogenic differentiation of MSC’s[7]. The high tunability of hyaluronic acid methacrylate allows it to be mixed with, and reinforce other types of hydrogels (such as collagen, or gelatin methacrylate)[8]. References: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5717235/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5627486/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5541838/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5447944/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615317/ https://www.ncbi.nlm.nih.gov/pubmed/21773726/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5460858/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748291/

PhotoHA™ is 100 mg of lyophilized methacrylated hyaluronic acid (HAMA). PhotoHA™ provides 3D hyaluronic acid hydrogels with the unique attributes to be prepared at various concentrations and photocrosslinked (requires a photoinitiator) to provide various gel stiffness.

Product Description Advanced BioMatrix offers PhotoHA™, a methacrylated hyaluronic acid (HAMA) for photocrosslinkable hydrogels. These hydrogels provide native-like 3D HA gels with the unique attributes to be prepared at various concentrations and photocrosslinked to provide various gel stiffness. Item Catalog Number Photoinitiator Included PhotoHA #5212 N/A PhotoHA + Irgacure #5220 Irgacure (365nm) PhotoHA + LAP #5274 LAP (405nm) PhotoHA + Ruthenium #5275 Ruthenium (400-450) Hyaluronic acid is the most abundant glycosaminoglycan in the body being an important component of several tissues throughout the body. While it is abundant in extracellular matrices, hyaluronic acid also contributes to tissue hydrodynamics, movement and proliferation of cells, and participates in a number of cell surface receptor interactions. For the majority of cell types, it is recommended to add additional ECM proteins to the hyaluronic acid hydrogels. The proteins provide important cell binding sites. Once solubilized, the PhotoHA™ can be stored at 2-10°C for 1 month. Parameter, Testing, and Method Methacrylated Hyaluronic Acid #5212 Sterilization Method Filtration Sterility - USP modified No growth Form Lyophilized Powder Package Size 100 mg Storage Temperature -20°C Shelf Life Minimum of 6 months from date of receipt Shelf Life After Reconstitution 1 Month Degree of Methacrylation 45-65% Molecular Weight 100-150 kDa NMR Analysis Characteristic Hydrogel Young's Modulus E (Pa) Characteristic Swelling Characteristics of PhotoHA™: 50 μL hydrogels fabricated in 4.7 mm diameter molds were imaged before and after incubation in phosphate buffered saline at 25°C for 24 hours. The diameter of hydrogels were quantified using ImageJ software. Statistical comparisons between groups (n=3) were performed via one-way ANOVA with post hoc testing and significance determined at p < 0.05. Compressive Modulus of PhotoHA™: Dynamic mechanical analysis (Q800, TA Instruments) was performed on 50 μL hydrogels fabricated in 4.7 mm diameter molds. Hydrogels were secured within a fluid cup via a 0.01 N pre-load and compressed to 30% strain at a rate of 0.5 N min-1. The Young’s modulus of each hydrogel was calculated as the slope of generated stress-strain curves between 10% and 20% strain. Statistical comparison between MeHA concentrations (n=3) was performed via Students t-test with two-tailed criteria and significance determined at p < 0.05. Reaction Behavior of PhotoHA™: Rheological time sweeps (AR2000 stress controlled rheometer, TA Instruments; 0.5% strain, 1 Hz, 25°C) of MeHA crosslinking with exposure to UV light (=320-390 nm) and in the presence of 0.05 wt% Irgacure 2959 (I2959). After 1 minute, the macromer solution (i.e. MeHA and I2959) was exposed to UV light, resulting in the plateau of moduli before 5 minutes.