Product Applications Read our HyStem eBrochure Here or Click on the title of the desired protocol to learn more: HyStem Hydrogel Stiffness/Rheology Curves 2D Cell Growth on HyStem Hydrogels HyStem 3D Cell Encapsulation for Cell Delivery Applications Guide HyStem 3D Cell Encapsulation in hydrogels using 96-well plates HyStem 3D Cell Encapsulation in hydrogels using TC Inserts Enzyme Digestion of HyStem Hydrogels for Recovery of Encapsulated Cells Cell Recovery from Surface of HyStem Hydrogels HyStem Gelation Time Variation HyStem Stiffness Variation Protocol 12.5 mL Kit HyStem Stiffness Variation Protocol 7.5 mL Kit Fluorescent Labeling HyStem Hydrogels HyStem ECM Incorporation

HyStem-C is a 3D hydrogel kit utilizing thiolated-hyaluronic acid (HA) and gelatin. HA is the simplest glycosaminoglycan (a negatively charged, linear, non-sulfated polysaccharide) that provides compression strength, lubrication, and hydration. HA regulates cell adhesion, motility, proliferation and differentiation.

HyStem-C is a 3D hydrogel kit utilizing thiolated-hyaluronic acid (HA) and gelatin. HA is the simplest glycosaminoglycan (a negatively charged, linear, non-sulfated polysaccharide) that provides compression strength, lubrication, and hydration. HA regulates cell adhesion, motility, proliferation and differentiation.

Product Description HyStem ® -C Hydrogel Kits - The starter matrix. HyStem ® -C hydrogels provide an excellent starting point for optimizing the matrix for cell culture. HyStem-C is fully chemically-defined and based on three biocompatible components: thiol-modified hyaluronan (Glycosil), thiol-reactive crosslinker, PEGDA (Extralink), and thiol-modified denatured collagen (Gelin-S ® ). Gelin-S provides basic cell-attachment sites for a wide variety of primary cells and cell lines and is therefore recommended as an ideal substrate for adherent cell types and for cell culture optimization. In some cases, HyStem-C hydrogels can be further enhanced by the addition of ECM proteins to match native signals. Product may be shipped ambient, but please store components at the temperature stated on the vials until ready for use. Features Hydrogels are suitable for culturing primary cells, stem cells and cell lines. Cells can be encapsulated or grown on the hydrogel surface in any format, including culture flasks, 6- to 384-well plates or tissue culture inserts. Hydrogels can be easily customized by the user to possess the desired stiffness and gelation time by manipulating component concentration and mixing ratios. Customizable gelation properties including gelation time and hydrogel stiffness. Gelation Reconstituted HyStem-C components remain liquid at 15 to 37°C. The hydrogel is formed when the crosslinking agent, Extralink ® (PEGDA) is added to a mixture of Glycosil ® (thiol-modified hyaluronan) and Gelin-S ® (thiol-modified gelatin). Gelation occurs in about twenty minutes after all three components are mixed. No steps depend on low temperatures or low pH. Diluting the components with phosphate-buffered saline (PBS) or cell-culture medium can increase the gelation time. 3D Cell Recovery Matrix For digesting the matrix and recovering cells, we recommend using a Collagenase/Hyaluronidase mixture from Stemcell Technologies found here.