Product Applications Read our HyStem eBrochure Here or Click on the title of the desired protocol to learn more: HyStem Hydrogel Stiffness/Rheology Curves 2D Cell Growth on HyStem Hydrogels HyStem 3D Cell Encapsulation for Cell Delivery Applications Guide HyStem 3D Cell Encapsulation in hydrogels using 96-well plates HyStem 3D Cell Encapsulation in hydrogels using TC Inserts Enzyme Digestion of HyStem Hydrogels for Recovery of Encapsulated Cells Cell Recovery from Surface of HyStem Hydrogels HyStem Gelation Time Variation HyStem Stiffness Variation Protocol 12.5 mL Kit HyStem Stiffness Variation Protocol 7.5 mL Kit Fluorescent Labeling HyStem Hydrogels HyStem ECM Incorporation

HyStem-HP is a 3D hydrogel kit utilizing thiolated-hyaluronic acid (HA), gelatin and heparin. The gelatin provides additional cell attachment sites, while the heparin ensures growth factor release at the immediate proximity of cells.

HyStem-HP is a 3D hydrogel kit utilizing thiolated-hyaluronic acid (HA), gelatin and heparin. The gelatin provides additional cell attachment sites, while the heparin ensures growth factor release at the immediate proximity of cells.

Product Description HyStem ® -HP Hydrogel Kit - The growth factor delivery matrix HyStem-HP hydrogel is fully chemically-defined and is ideal for cell applications whereby the slow, continuous release of growth factors is crucial to re-creating a desired microenvironment. The HyStem-HP Hydrogel Kit contains a combination of thiol-modified hyaluronan and a thiol-modified heparin (Heprasil ® ), thiol-modified denatured collagen (Gelin-S), and thiolreactive crosslinker, PEGDA (Extralink). HA is the simplest glycosaminoglycan (a negatively charged, linear, non-sulfated polysaccharide) that provides compression strength, lubrication, and hydration. HA regulates cell adhesion, motility, proliferation and differentiation. The immobilized heparin in the HyStem-HP hydrogel mimics the heparin sulfate proteoglycans normally present in the extracellular matrix. Heparin forms an ionic bond with proteins and protects them from proteolysis and facilitates their slow release into the cell culture medium. This significantly reduces the amount of growth factor required to trigger cell growth or differentiation compared to when growth factors are added directly to the medium. Product may be shipped ambient, but please store components at the temperature stated on the vials until ready for use. Features Growth factors can be mixed into the hydrogels prior to gelation to provide a slow growth factor release depot. Hydrogels are suitable for animal implantation (such as angiogenesis applications and cell or drug delivery), culturing of primary cells, stem cells, and cell lines in the presence of growth factors. Cells can be encapsulated or grown on the hydrogel surface in any format, including culture flasks, 6- to 384-well plates or tissue culture inserts. Hydrogels can be easily customized by the user to possess the desired stiffness and gelation time by manipulating component concentration and mixing ratios. Gelation Reconstituted HyStem-HP components remain liquid at 15 to 37°C. The hydrogel is formed when the crosslinking agent, Extralink ® (PEGDA) is added to a mixture of Heprasil ® (thiol-modified hyaluronan plus heparin) and Gelin-S ® (thiol-modified gelatin). Gelation occurs in about twenty minutes after all three components are mixed. No steps depend on low temperatures or low pH. Diluting the components with phosphate-buffered saline (PBS) or cell-culture medium can increase the gelation time. 3D Cell Recovery Matrix For digesting the matrix and recovering cells, we recommend using a Collagenase/Hyaluronidase mixture from Stemcell Technologies found here.