Product Applications Matrigel Alternatives for Neuronal Assays Matrigel Alternatives for Migration and Wound Healing Assays

This Vitronectin solution has been purified from human plasma where it is found as a mixture of 75kDa and 65kDa polypeptides. Vitronectin’s primary use in cell culture is related to cell adhesion. It also binds to heparin and collagen.

Product Description Vitronectin is a monomeric glycoprotein used to promote cell attachment, migration, proliferation and differentiation in a broad number of cell lines and types. This product has been purified from human plasma where it is found as a mixture of 75kDa and 65kDa polypeptides. Vitronectin’s primary use in cell culture is related to cell adhesion. It also binds to heparin and collagen. Vitronectin is ideal for coating of surfaces. The optimal concentration for cell attachment and culture may differ for various cell types. Vitronectin has been used at a final coating concentration as low as 50 ng/cm2 on plasticware. It is provided in user-friendly packaging for use and storage. Vitronectin is sterile filtered and is supplied as a ready to use solution after thawing and concentration adjustment. This product is shipped separately on dry ice. Parameter, Testing, and Method Vitronectin #5051 Quantity 0.1 mg or 0.5 mg Volume 0.2 mL or 1 mL Concentration 0.5 mg/mL Purity - SDS PAGE Electrophoresis > 95% Formulation 0.15 M NaCl, 0.005M HEPES pH 7.4 Form Solution Source Human, Plasma Storage Temperature -20°C or -70°C for long term storage Shelf Life Minimum of 6 months from date of receipt Sterilization Method Filtration Cell Attachment Assay Passes Sterility - USP modified No growth Safety Source material found negative for infectious agents