Product Applications Read our PhotoHA-Soft Photocrosslinking Rheology White Paper here. Read our Methacrylated Extracellular Matrices eBrochure Here PhotoHA™ Methacrylated Hyaluronic acid can be used to form hydrogels for ex-vivo engineering of autologous cartilage tissue[1] or as a mesenchymal stem cell carrier in cartilage repair[2]. Because the stiffness can be widely adjusted by altering concentration or UV-light exposure, methacrylated HA has been used to measure the effects of matrix stiffness on cell phenotype and function[3][4]. Methacrylated HA can be used for 3D bioprinting (extrusion[5], inkjet[5] and photolithographic[6]) to create structures that promote osteogenic differentiation of MSC’s[7]. The high tunability of hyaluronic acid methacrylate allows it to be mixed with, and reinforce other types of hydrogels (such as collagen, or gelatin methacrylate)[8]. References: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5717235/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5627486/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5541838/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5447944/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5615317/ https://www.ncbi.nlm.nih.gov/pubmed/21773726/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5460858/ https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5748291/

PhotoHA™-INK (soft) is 5 mL of a 4% solution of methacrylated hyaluronic acid (GM-HA) in PBS with 0.5% LAP. PhotoHA™ (soft) is a GM-HA which provides softer hydrogels than the (stiff) HAMA. This ink is pre-formulated and ready for cell printing in the BIONOVA X.

Product Description Advanced BioMatrix offers PhotoHA™-INK, 5 mL of methacrylated hyaluronic acid (GM-HA) pre-formulated for photocrosslinkable hydrogels with the BIONOVA X DLP 3D Bioprinter. The product is a 4% solution with 0.5% LAP. Hyaluronic acid is the most abundant glycosaminoglycan in the body being an important component of several tissues throughout the body. While it is abundant in extracellular matrices, hyaluronic acid also contributes to tissue hydrodynamics, movement and proliferation of cells, and participates in a number of cell surface receptor interactions. For the majority of cell types, it is recommended to add additional ECM proteins to the hyaluronic acid hydrogels. The proteins provide important cell binding sites. Parameter, Testing, and Method PhotoHA™-INK (Soft) Sterilization Method Filtration Sterility - USP modified No growth Form Sterile Solution Package Size 5 mL Storage Temperature 2-8°C Shelf Life Minimum of 6 months from date of receipt Degree of Substitution 25-45% Molecular Weight 100-150 kDa Source Recombinant Hydrogel Young's Modulus E (Pa) Characteristic