Product Applications Read our HyStem eBrochure Here or Click on the title of the desired protocol to learn more: 2D Cell Growth on HyStem Hydrogels HyStem 3D Cell Encapsulation for Cell Delivery Applications Guide HyStem 3D Cell Encapsulation in hydrogels using 96-well plates HyStem 3D Cell Encapsulation in hydrogels using TC Inserts Enzyme Digestion of HyStem Hydrogels for Recovery of Encapsulated Cells Fluorescent Labeling of HyStem Hydrogels Cell Recovery from Surface of HyStem Hydrogels HyStem ECM Incorporation HyStem Gelation Time Variation HyStem Stiffness Variation Protocol for 7.5 mL kit HyStem Stiffness Variation Protocol for 12.5 mL kit

PEGDA-INK is 10mL of preformulated 50% PEGDA ink (700 MW) with 0.5% LAP that gels rapidly using the BIONOVA X DLP 3D Bioprinter.

Product Description Polyethylene Glycol Diacrylate (PEGDA) hydrogels are powerful tools for uncovering basic cellular biology because they are considered biologically inert (“blank slate”) and their mechanical properties can be varied over a large range of moduli. PEGDA is an emerging scaffold for tissue engineering and regenerative medicine since polymerization can occur rapidly at room temperature and requires low energy input, has high water content, is elastic, and can be customized to include a variety of biological molecules. PEGDA is a crosslinker repeating polymer crosslinker and is >80% acrylated. It can be used as a thiol-reactive crosslinker in our HyStem systems at low concentration, or can be used to make a PEGDA-only hydrogel in the presence of a free-radical chain photoinitiator and light source. PEG-only gels do not typically support cell attachment without the incorporation of cellular attachment sites. Applications Regular and 3-D cell culture Tissue engineering Photolithography Parameter, Testing, and Method PEGDA-INK Sterilization Method Filtration Sterility - USP modified No growth Form Sterile Solution Package Size 10 mL Storage Temperature 2-8°C Shelf Life Minimum of 6 months from date of receipt Molecular Weight 700 kDa Source Synthetic Hydrogel Young's Modulus E (Pa) Characteristic