Product Applications Read our HyStem eBrochure Here or Click on the title of the desired protocol to learn more: HyStem Hydrogel Stiffness/Rheology Curves 2D Cell Growth on HyStem Hydrogels HyStem 3D Cell Encapsulation for Cell Delivery Applications Guide HyStem 3D Cell Encapsulation in hydrogels using 96-well plates HyStem 3D Cell Encapsulation in hydrogels using TC Inserts Enzyme Digestion of HyStem Hydrogels for Recovery of Encapsulated Cells Cell Recovery from Surface of HyStem Hydrogels HyStem Gelation Time Variation HyStem Stiffness Variation Protocol 12.5 mL Kit HyStem Stiffness Variation Protocol 7.5 mL Kit Fluorescent Labeling HyStem Hydrogels HyStem ECM Incorporation

Gelin-S^® is a thiol-modified gelatin that comes as a lyophilized powder. Gelin-S^® is a component of the HyStem^®-C and HyStem^®-HP kits, but can be purchased separately here. Comes with 10 mL of Buffer B.

Gelin-S^® is a thiol-modified gelatin that comes as a lyophilized powder. Gelin-S^® is a component of the HyStem^®-C and HyStem^®-HP kits, but can be purchased separately here. Comes with 10 mL of Buffer B.

Gelin-S ® is a thiol-modified gelatin that comes as a lyophilized powder. Gelin-S ® is a component of the HyStem ® -C and HyStem ® -HP kits, but can be purchased separately here. Comes with 10 mL of Buffer B.

Product Description Gelin-S ® is thiol-modified gelatin (denatured collagen) and is a component of the HyStem ® -C, and HyStem ® -HP hydrogel kits. HyStem are reconstituted with a buffered 1X PBS. The resulting components will also result in a pH neutral isotonic salt hydrogel. Most cells do not grow well on Gelin-S ® –only hydrogels. Instead, Gelin-S ® should be used in conjunction with Glycosil ® (thiol-modified hyaluronic acid) or Heprasil ® (thiol-modified hyaluronic acid with thiol-modified heparin). Reconstituted Gelin-S remains liquid at 15 to 37°C. The gelatin used to make Gelin-S ® is from Type A Gelatin, Bloom 250, derived from porcine skin. Gelin-S ® (thiol-modified gelatin) is packaged in 5.0 mL vials containing 50mg. Vials are blanketed by nitrogen and under a slight vacuum. Store Gelin-S ® in the original vial, unopened, at -20 °C for up to one year. Do not uncap the Gelin-S ® vials since they will crosslink in the presence of oxygen. Use a syringe and needle to add 1X PBS to the vial. Note: It is recommended to reconstitute each vial in its entirety.