Product Applications Read our HyStem eBrochure Here or Click on the title of the desired protocol to learn more: HyStem Hydrogel Stiffness/Rheology Curves 2D Cell Growth on HyStem Hydrogels HyStem 3D Cell Encapsulation for Cell Delivery Applications Guide HyStem 3D Cell Encapsulation in hydrogels using 96-well plates HyStem 3D Cell Encapsulation in hydrogels using TC Inserts Enzyme Digestion of HyStem Hydrogels for Recovery of Encapsulated Cells Cell Recovery from Surface of HyStem Hydrogels HyStem Gelation Time Variation HyStem Stiffness Variation Protocol 12.5 mL Kit HyStem Stiffness Variation Protocol 7.5 mL Kit Fluorescent Labeling HyStem Hydrogels HyStem ECM Incorporation

Heprasil^® is a mixture of thiol-modified hyaluronic acid thiol-modified heparin. Heprasil^® is a component of the HyStem^®-HP kits, but can be purchased separately here. Comes with 10 mL of Buffer A.

Heprasil^® is a mixture of thiol-modified hyaluronic acid thiol-modified heparin. Heprasil^® is a component of the HyStem^®-HP kits, but can be purchased separately here. Comes with 10 mL of Buffer A.

Heprasil ® is a mixture of thiol-modified hyaluronic acid thiol-modified heparin. Heprasil ® is a component of the HyStem ® -HP kits, but can be purchased separately here. Comes with 10 mL of Buffer A.

Product Description Heprasil ® is thiol-modified hyaluronic acid with thiol-modfied heparin and is a component of the HyStem ® hydrogel kit. HyStem ® components are reconstituted with a buffered 1X PBS. The resulting components will also result in a pH neutral isotonic salt hydrogel. Hyaluronic acid is a major constituent of native extracellular matrix (ECM). Heparin is also present in the ECM as heparan sulfate. Most cells do not attach to Heprasil-only hydrogels. Heprasil ® must be used in conjunction with Gelin-S ® or ECM proteins such as laminin, collagen, or fibronectin for most 3-D cell culture and tissue-engineering applications. Gelation Reconstituted Heprasil ® remains liquid at 15 to 37°C. Without a crosslinker, Heprasil ® will form a hydrogel via disulfide bond formation; however, the gelation time is over twenty-four hours. If Extralink ® is used to crosslink the Heprasil ® , the gelation time is about twenty minutes with no low-temperature or low-pH steps. Diluting Heprasil ® with phosphate-buffered saline (PBS) or cell-culture medium can increase its gelation time.