Product Applications Read our HyStem eBrochure Here or Click on the title of the desired protocol to learn more: HyStem Hydrogel Stiffness/Rheology Curves 2D Cell Growth on HyStem Hydrogels HyStem 3D Cell Encapsulation for Cell Delivery Applications Guide HyStem 3D Cell Encapsulation in hydrogels using 96-well plates HyStem 3D Cell Encapsulation in hydrogels using TC Inserts Enzyme Digestion of HyStem Hydrogels for Recovery of Encapsulated Cells Cell Recovery from Surface of HyStem Hydrogels HyStem Gelation Time Variation HyStem Stiffness Variation Protocol 12.5 mL Kit HyStem Stiffness Variation Protocol 7.5 mL Kit Fluorescent Labeling HyStem Hydrogels HyStem ECM Incorporation

Extralink^® PEGDA is a thiol-reactive crosslinker designed for use in HyStem^®-C and HyStem^®-HP kits. Pack comes with Buffer B.

Extralink^® PEGDA is a thiol-reactive crosslinker designed for use in HyStem^®-C and HyStem^®-HP kits. Pack comes with Buffer B.

Extralink ® PEGDA is a thiol-reactive crosslinker designed for use in HyStem ® -C and HyStem ® -HP kits. Pack comes with Buffer B.

Product Description Extralink ® (PEGDA, polyethylene glycol diacrylate) 0.5mL vials contain 5mg of diacrylated PEG and 2.5 mL vials contain 25 mg. Vials are blanketed by nitrogen and under a slight vacuum. Each pack comes with sufficient Buffer B, at no additional cost. Extralink ® is polyethylene diacrylate (PEGDA) with a molecular weight of 3500 Da. It is a thiol-reactive crosslinker that covalently reacts with the thiol groups in Glycosil ® , Heprasil ® and Gelin-S ® to form viscoelastic hydrogels. Extralink ® vials are >80% acrylated and supplied as the crosslinking component in all HyStem ® hydrogel kits. Extralink ® vials are paired with HyStem ® -C and HyStem ® –HP kits while Extralink ® -Lite vials are paired with HyStem ® hydrogel kits to provide comparable experiences despite the different HA concentrations. Gelation Extralink ® is used to chemically crosslink Glycosil ® (or Heprasil ® ) and Gelin-S ® in Hystem ® -C and HyStem ® -HP kits. The gelation time ranges from as short as 5 minutes to as long as a couple of hours, depending upon the amount of Extralink used and the concentration/dilution of the Glycosil ® , Gelin-S ® and/or Heprasil ® . Similarly, Extralink ® -Lite is used in place of Extralink ® for applications where Gelin-S ® is not included. The higher relative concentration of Glycosil ® requires less crosslinker to emulate the gelation time and stiffness of HyStem ® -C and HyStem ® -HP. STORAGE Extralink: Store Extralink in the original vial unopened at -20°C for up to one year. Reconstituted Extralink solutions can be stored at -20 °C for ~ one month. Note: It is recommended to reconstitute each vial in its entirety.