Product Applications Matrigel Alternatives for Neuronal Assays Matrigel Alternatives for Migration and Wound Healing Assays

Poly-L-Lysine comes with 50 mL of a 0.1 mg/ml sterile-filtered solution. Poly-L-Lysine is a synthetic amino acid chain that is positively charged and widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. This product’s molecular weight ranges from 70,000 to 150,000 Da.

Product Description Poly-L-Lysine is a synthetic amino acid chain that is positively charged having one hydrobromide per unit of Lysine. Poly-L-Lysine is widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. This molecule has been used to culture a wide variety of cell types. Certain cell types secrete proteases, which can digest Poly-L-Lysine. For those cell types, Poly-D-Lysine, Catalog # 5049 should be used. The molecular weight of Poly-L-Lysine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment. Poly-L-Lysine surface coatings are designed to improve cell attachment, growth and differentiation of many cell types. Coated surfaces will often improve cell attachment in reduced or serum-free conditions. This product is supplied in a sterile 50 ml package size at a concentration of 0.1 mg/ml. Parameter, Testing, and Method Poly-L-Lysine #5048 Form Solution Package Size 50 mL Storage Temperature 2-10°C Sterilization Method Filtration Molecular Weight 70,000 - 150,000 Da Sterility - USP modified No Growth Cell Attachment Assay Pass Source Synthetic Shelf Life Minimum of 6 months from date of receipt Concentration 0.1 mg/mL