Product Applications Cytotoxicity and drug screening assays Live/dead discrimination in 2D and 3D cultures Hydrogel and ECM-based cell viability studies High-content imaging Flow cytometry viability analysis Tissue engineering and bioprinting validation

Dual-color cell viability assay combining Calcein AM and Propidium Iodide for rapid, high-contrast viability assessment. Cytus™ Green labels metabolically active cells, while Cytus™ Red stains dead cells, enabling accurate imaging, cytotoxicity studies, and quantitative analysis.

Product Description The Cytus™ Green / Red Cell Viability Kit is a dual-color fluorescence assay designed for rapid and reliable assessment of live and dead cells. It combines Cytus™ Green Calcein AM for viable cells with Cytus™ Red Propidium Iodide for non-viable cells, enabling simultaneous discrimination within mixed populations. Cytus™ Green is a membrane-permeable, non-fluorescent compound that is enzymatically converted into fluorescent calcein in metabolically active cells. This conversion requires intact membranes and intracellular esterase activity, resulting in strong green fluorescence proportional to viable cell number. Cytus™ Red selectively stains dead or membrane-compromised cells by intercalating into nucleic acids, producing bright red fluorescence upon binding. Together, these dyes provide a robust and widely validated method for viability assessment across a range of experimental systems. Parameter, Testing, and Method Cytus™ Green / Red #5471 Live Cell Dye Green (Cytus™ Green Calcein AM) Dead Cell Dye Red (Cytus™ Red Propidium Iodide) Stock Concentration Green/Red: 1 mM in DMSO Stock Volume Green/Red: 100 µL Appearance Green: yellow hue, Red: red hue; clear liquid Sterility Non-Sterile Assay Type Live dead cell viability Cell Compatibility Live and dead cells Working Concentration Green: 2 µM; Red: 2 µM Excitation/Emission (Green) 501 / 521 nm Excitation/Emission (Red) 537 / 617 nm Storage Temperature -20°C Shelf Life Minimum of 6 months from date of receipt