Product Applications Cell viability + nuclear staining in a single assay 3D cell culture and hydrogel viability analysis Drug screening and cytotoxicity studies High-content imaging (HCS) Cell counting and normalization Tissue engineering and bioprinting validation ECM interaction studies (collagen, HA, GelMA systems)

All-in-one fluorescence kit for cell viability and nuclear staining. Combines Calcein AM, Propidium Iodide, and Hoechst-33342 Cytus™ Blue for simultaneous visualization of viable cells, dead cells, and nuclei. Ideal for imaging, screening, and 3D cell culture workflows.

Product Description The Cytus™ RGB Cell Viability and Nucleus Stain Kit is a comprehensive, three-color fluorescence assay that enables simultaneous visualization of live cells, dead cells, and nuclei. The kit combines Cytus™ Green (Calcein AM), Cytus™ Red (Propidium Iodide), and Cytus™ Blue (Hoechst 33342) to provide a complete picture of cell health and population structure within a single experiment. Cytus™ Green labels metabolically active cells through esterase-mediated conversion to fluorescent calcein, while Cytus™ Red selectively stains membrane-compromised cells by binding nucleic acids. Cytus™ Blue binds DNA and provides high-contrast nuclear labeling in both live and fixed cells. Together, these dyes deliver strong signal separation across green, red, and blue channels, enabling robust qualitative imaging and quantitative analysis across microscopy, plate-based assays, and flow cytometry workflows. Parameter, Testing, and Method Cytus™ RGB #5470 Live Cell Dye Green (Cytus™ Green Calcein AM) Dead Cell Dye Red (Cytus™ Red Propidium Iodide) Nuclear Dye Blue (Cytus™ Blue Hoechst-33342) Stock Concentration Green/Red: 1 mM in DMSO; Blue: 1 mM in water Stock Volume Green/Red: 100 µL; Blue: 100 µL Appearance Green: yellow hue, Red: red hue, Blue: green hue; clear liquid Sterility Non-Sterile Assay Type Cell Viability + Nuclear (3-color) Cell Compatibility Live, dead and fixed cells Working Concentration Green: 2 µM; Red: 2 µM; Blue: 1–5 µM Excitation/Emission (Green) 501 / 521 nm Excitation/Emission (Red) 537 / 617 nm Excitation/Emission Blue ~350 / 454 nm Storage Temperature -20°C Shelf Life Minimum of 6 months from date of receipt