Product Applications Cell adhesion assays Proliferation and endpoint cell quantification Biomaterial and ECM coating evaluation (PureCol®, GelMA, etc.) Mechanobiology and stiffness studies (CytoSoft™ plates) High-throughput screening (96-well and higher formats) Cell spreading and morphology analysis

Cytus™ Violet is a crystal violet-based stain for quantifying adherent cells in adhesion and proliferation assays. Provides strong, uniform staining for imaging or plate-based quantification, enabling reliable evaluation of cell attachment on biomaterials, ECM coatings, and stiffness-tunable surfaces.

Product Description Cytus™ Violet Cell Adhesion Stain is a ready-to-use crystal violet-based staining solution designed for visualization and quantification of adherent cells. The dye binds strongly to cellular DNA and proteins, producing an intense, uniform stain that highlights attached cells while nonadherent cells are removed during washing. This enables clear evaluation of cell attachment, spreading, and adhesion performance across a wide range of substrates, including tissue culture plastics, extracellular matrix coatings, and stiffness-tunable biomaterials such as CytoSoft™. The workflow is simple and reproducible, supporting both imaging-based analysis and quantitative plate-based measurements. When combined with automated imaging or plate readers, Cytus™ Violet enables efficient, high-content analysis of cell adhesion across multiple conditions. Parameter, Testing, and Method Cytus™ Violet #5466 Dye Type Crystal Violet Stock Concentration 0.1% w/v in water Stock Volume 2 x 25 mL Appearance Dark purple liquid Sterility Sterile Assay Type Cell Adhesion Compatibility Binds strongly to DNA and proteins Working Concentration 0.1% w/v Working Volume / Well (Plate: 96-well) 100 to 200 µL Detection Methods Brightfield and fluorescence Absorbance ~590 nm Fluorescence Excitation/Emission (optional) ~592/636 nm Storage Room temperature (25°C) Shelf Life Minimum of 6 months from date of receipt